G3: Genes, Genomes, Genetics ( IF 2.6 ) Pub Date : 2020-10-27 , DOI: 10.1534/g3.120.401656 Calvin Mok 1 , Gabriella Belmarez 2 , Mark L Edgley 3 , Donald G Moerman 3 , Robert H Waterston 2
Whether generated within a lab setting or isolated from the wild, variant alleles continue to be an important resource for decoding gene function in model organisms such as Caenorhabditis elegans. With advances in massively parallel sequencing, multiple whole-genome sequenced (WGS) strain collections are now available to the research community. The Million Mutation Project (MMP) for instance, analyzed 2007 N2-derived, mutagenized strains. Individually, each strain averages ~400 single nucleotide variants amounting to ~80 protein-coding variants. The effects of these variants, however, remain largely uncharacterized and querying the breadth of these strains for phenotypic changes requires a method amenable to rapid and sensitive high-throughput analysis. Here we present a pooled competitive fitness approach to quantitatively
中文翻译:
PhenoMIP:通过分子倒置探针对多种秀丽隐杆线虫种群进行高通量表型分析。
无论是在实验室环境中产生还是从野外分离,变异等位基因仍然是在模式生物(如秀丽隐杆线虫)中解码基因功能的重要资源. 随着大规模平行测序的进步,研究界现在可以使用多个全基因组测序 (WGS) 菌株集合。例如,百万突变项目 (MMP) 分析了 2007 年 N2 衍生的诱变菌株。单独地,每个菌株平均约 400 个单核苷酸变体,相当于约 80 个蛋白质编码变体。然而,这些变体的影响在很大程度上仍未得到表征,并且查询这些菌株的表型变化的广度需要一种适合快速和敏感的高通量分析的方法。在这里,我们提出了一种汇总的竞争健身方法来定量