A standard protocol was used to determine partition (K) and diffusion (D) coefficients in dermatomed human skin and isolated human skin layers for 50 compounds relevant to cosmetics ingredients. K values were measured in dermatomed skin, isolated dermis, whole epidermis, intact stratum corneum (SC), delipidized SC and SC lipids by direct measurements of the radioactivity in the tissue layers/lipid component vs. buffer samples. D determinations were made in dermatomed skin, isolated dermis, whole epidermis and intact SC using a non-linear regression of the cumulative receptor fluid content of radiolabeled compound, fit to the solution of Fick's 2nd Law. Correlation analysis was completed between K, D, and physicochemical properties. The amount of interindividual (donor) and intraindividual (replicate) variability in the K and D data was characterized for each skin layer and chemical. These data can be further used to help inform the factors that influence skin bioavailability and to help improve in silico models of dermal penetration.

使用标准方案来确定与化妆品成分相关的 50 种化合物在人体皮肤和分离的人体皮肤层中的分配 (K) 和扩散 (D) 系数。通过直接测量组织层/脂质成分与缓冲液样品中的放射性，在切皮皮肤、分离的真皮、完整表皮、完整角质层 (SC)、脱脂 SC 和 SC 脂质中测量 K 值。使用放射性标记化合物的累积受体流体含量的非线性回归，在皮肤切开的皮肤、分离的真皮、完整的表皮和完整的皮下进行 D 测定，符合菲克第二定律的解决方案。完成K、D和理化性质之间的相关分析。K 和 D 数据中的个体间（供体）和个体内（重复）变异量的特点是针对每个皮肤层和化学物质。这些数据可进一步用于帮助了解影响皮肤生物利用度的因素，并帮助改进皮肤渗透的计算机模型。