The airway mucosa is the first point of exposure to all inhalant materials. It is possible to produce microenvironments functionally similar to airway by using different types of extracellular matrices around mucosal cells, such as an extracellular matrix decellularized from trachea. In this study, we introduced a novel human respiratory mucosa-on-a-chip model that uses decellularized tracheal extracellular matrix to assess functional similarities. We also evaluated the effects of respiratory toxicant based on changes in cell viability, tight junction function, oxidative stress, expression of cell adhesion proteins in the vascular network, mucous secretion, and inflammatory cytokine secretion after treatment. This novel human respiratory mucosa-on-a-chip model is expected to be useful for assessing respiratory mucosa reactions to external bacteria or viruses and inhaled drugs.

气道粘膜是所有吸入材料的第一暴露点。通过在粘膜细胞周围使用不同类型的细胞外基质（例如从气管中脱细胞的细胞外基质），可以产生功能类似于气道的微环境。在这项研究中，我们介绍了一种新型的人呼吸片上粘膜模型，该模型使用脱细胞的气管细胞外基质来评估功能相似性。我们还根据治疗后细胞活力，紧密连接功能，氧化应激，血管网络中细胞粘附蛋白的表达，黏液分泌和炎性细胞因子分泌的变化评估了呼吸道有毒物质的作用。