In this study, an optical platform based on fluorescent nanofiber was developed for detection of microRNA-21 as a biomarker of cancerous cells. The fluorescent polyacrylonitrile nanofiber was fabricated using electrospinning method. The surfaces of nanofiber were treated by NaOH to convert nitrile groups to carboxyl moieties. Activation of these carboxyl groups by EDC-sulfo NHS coupling agents was done and then the covalent bonding between COOH groups of nanofiber and –NH 2 groups of amino-probe was formed. The results of SEM, XRD, FTIR, and fluorescence microscopy confirmed that the process of fluorescent nanofiber synthesis was successfully performed. Hybridization of probe and microRNA-21 showed that the capture efficiency of this fluorescent nanofiber was 1 pmol μ l −1 and these nanofibers have the potential to be used as optical sensors for detection of microRNA-21.

中文翻译：

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新型荧光聚丙烯腈电纺纳米纤维的制备，用于基于DNA的microRNA-21光学生物传感。

在这项研究中，开发了一种基于荧光纳米纤维的光学平台，用于检测作为癌细胞生物标志物的microRNA-21。采用静电纺丝法制备了荧光聚丙烯腈纳米纤维。用NaOH处理纳米纤维的表面以将腈基转化为羧基部分。用EDC-磺基NHS偶联剂激活了这些羧基，然后在纳米纤维的COOH基和氨基探针的-NH 2基之间形成了共价键。SEM，XRD，FTIR和荧光显微镜的结果证实，荧光纳米纤维的合成过程成功进行。