Pakistan is one of the top five date fruit-producing countries and produced more than 30% wastes in picking, packing, storage, and commercialization stages. The date fruit wastes are usually considered inedible for humans and only used for livestock feed. In current research, Bacillus licheniformis KIBGE-IB3 was screened for pectinase, xylanase, cellulase, and amylase production using date fruit wastes as substrate through solid state fermentation. The B. licheniformis KIBGE-IB3 produced higher concentration of pectinase using date fruit wastes as substrate as compared to amylase, cellulase, and xylanase. B. licheniformis KIBGE-IB3 produced maximum pectinase using 5.0 g/dl date fruit wastes and 0.5 g/dl yeast extract. B. licheniformis KIBGE-IB3 required pH 7.0, 37 °C incubation temperature, and 72 h incubation period for maximum production of pectinase. It has been concluded that date fruit waste is a good source of biomass and can be utilized for the commercial production of pectinase.

中文翻译：

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使用枣果渣作为底物，从地衣芽孢杆菌KIBGE-IB3生产商业上重要的酶。

巴基斯坦是五个最大的枣果生产国之一，在采摘，包装，储存和商业化阶段产生了30％以上的废物。枣果废弃物通常被认为是人类不可食用的，仅用于牲畜饲料。在当前的研究中，以枣果渣为固态发酵物，筛选了地衣芽孢杆菌KIBGE-IB3的果胶酶，木聚糖酶，纤维素酶和淀粉酶生产。与枣淀粉酶，纤维素酶和木聚糖酶相比，地衣芽孢杆菌KIBGE-IB3使用枣果渣作为底物产生了更高浓度的果胶酶。地衣芽孢杆菌KIBGE-IB3使用5.0 g / dl的枣果渣和0.5 g / dl的酵母提取物产生了最大的果胶酶。地衣芽孢杆菌KIBGE-IB3需要pH 7.0、37°C​​孵育温度，孵育72小时可最大程度地产生果胶酶。已经得出的结论是，枣类水果废料是生物质的良好来源，可用于果胶酶的商业生产。