Introduction:

Oxidative stress is a key contributor to aging and age-related diseases. In the present study, we examine the protective effects of PFT, a novel kefir product, against age-associated oxidative stress using aged (10-month-old) mice.

Methods:

Mice were treated with PFT orally at a daily dose of 2 mg/kg body weight over 6 weeks, and antioxidant status, protein oxidation, and lipid peroxidation were studied in the brain, liver, and blood.

Results:

PFT supplementation significantly reduced the oxidative stress biomarkers malondialdehyde (MDA) and nitric oxide; reversed the reductions in glutathione (GSH) levels, total antioxidant capacity (TAC), and anti-hydroxyl radical (AHR) content; enhanced the antioxidant enzyme activities of glutathione peroxidase (GPx), catalase (CAT), and superoxide dismutase (SOD); inhibited the liver enzyme levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT); significantly reduced triglyceride (TG), total cholesterol (TC), and low density lipoprotein (LDL) levels; and significantly elevated high density lipoprotein (HDL) levels. Interestingly, PFT supplementation reversed the oxidative changes associated with aging, thus bringing levels to within the limits of the young control mice in the brain, liver, and blood. We also note that PFT affects the redox homeostasis of young mice and that it is corrected post-treatment with PFT.

Conclusion:

Our findings show the effectiveness of dietary PFT supplementation in modulating age-associated oxidative stress in mice and motivate further studies of PFT’s effects in reducing age-associated disorders where free radicals and oxidative stress are the major cause.

中文翻译：

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通过 PFT 逆转小鼠与年龄相关的氧化应激，一种新的开菲尔产品。

介绍：

氧化应激是导致衰老和与年龄有关的疾病的关键因素。在本研究中，我们使用老年（10 个月大）小鼠检查了 PFT（一种新型开菲尔产品）对与年龄相关的氧化应激的保护作用。

方法：

小鼠在 6 周内以每日 2 mg/kg 体重的剂量口服 PFT，并研究了大脑、肝脏和血液中的抗氧化状态、蛋白质氧化和脂质过氧化。

结果：

PFT 补充剂显着降低了氧化应激生物标志物丙二醛 (MDA) 和一氧化氮；逆转谷胱甘肽 (GSH) 水平、总抗氧化能力 (TAC) 和抗羟基自由基 (AHR) 含量的降低；增强谷胱甘肽过氧化物酶 (GPx)、过氧化氢酶 (CAT) 和超氧化物歧化酶 (SOD) 的抗氧化酶活性；抑制天冬氨酸氨基转移酶（AST）和丙氨酸氨基转移酶（ALT）的肝酶水平；显着降低甘油三酯 (TG)、总胆固醇 (TC) 和低密度脂蛋白 (LDL) 水平；并显着提高高密度脂蛋白（HDL）水平。有趣的是，PFT 补充剂逆转了与衰老相关的氧化变化，从而使大脑、肝脏和血液中的年轻对照小鼠的水平达到了极限。

结论：

我们的研究结果表明膳食 PFT 补充剂在调节小鼠年龄相关氧化应激方面的有效性，并激发了进一步研究 PFT 在减少自由基和氧化应激是主要原因的年龄相关疾病方面的作用。