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Blockage of C-C Chemokine Ligand 2 Alleviated the Appendicitis-Induced Injury in Rabbit Model.
Journal of Interferon & Cytokine Research ( IF 2.3 ) Pub Date : 2020-09-17 , DOI: 10.1089/jir.2020.0050
Wen Liu 1 , Anrong Zeng 1 , Hanzhou Tang 1 , JinWei Qiang 1
Affiliation  

C-C chemokine ligand 2 (CCL2), a low-molecular-weight cytokine, is upregulated in inflammation-related diseases. However, the underlying function of CCL2 remains unknown in human appendicitis. The present study aimed to examine the role of CCL2 in appendicitis. An enzyme-linked immunosorbent assay was performed to examine the secretion of CCL2 in the peripheral blood of patients with simple and complex appendicitis, respectively. A flow cytometry assay was used to quantify the level of the CCL2 receptor, CCR2. Moreover, we constructed an appendicitis model in rabbits. Quantitative real time-polymerase chain reaction and Western blot were used to determine CCL2 and CCR2 levels in the appendicitis model. CCL2 antibodies were used to silence the endogenous activity of CCL2 in vivo. Magnetic resonance imaging and a histopathology assay were used to examine the appendicitis-induced injury in rabbits. Our results suggested that CCL2 and its main receptor CCR2 were upregulated in patients with appendicitis, particularly those with complex appendicitis (gangrenous and perforated appendicitis). Moreover, CCL2 silencing alleviated the appendicitis-induced injury in rabbits. Our findings not only illustrate the potential value of CCL2 as a biomarker in appendicitis diagnosing but also provide novel insight into appendicitis treatment.

中文翻译:

CC 趋化因子配体 2 的阻塞减轻了兔模型中阑尾炎引起的损伤。

CC 趋化因子配体 2 (CCL2) 是一种低分子量细胞因子,在炎症相关疾病中上调。然而,CCL2 在人类阑尾炎中的潜在功能仍然未知。本研究旨在检查 CCL2 在阑尾炎中的作用。分别采用酶联免疫吸附法检测单纯性和复杂性阑尾炎患者外周血中CCL2的分泌情况。流式细胞术测定用于量化 CCL2 受体 CCR2 的水平。此外,我们在兔子中构建了阑尾炎模型。定量实时聚合酶链反应和蛋白质印迹用于确定阑尾炎模型中的 CCL2 和 CCR2 水平。CCL2 抗体用于在体内沉默 CCL2 的内源活性磁共振成像和组织病理学测定用于检查兔阑尾炎引起的损伤。我们的研究结果表明,CCL2 及其主要受体 CCR2 在阑尾炎患者中上调,尤其是那些患有复杂性阑尾炎(坏疽和穿孔性阑尾炎)的患者。此外,CCL2 沉默减轻了兔阑尾炎引起的损伤。我们的研究结果不仅说明了 CCL2 作为阑尾炎诊断生物标志物的潜在价值,而且还为阑尾炎治疗提供了新的见解。
更新日期:2020-09-22
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