Abstract In recent years, green synthesis of nanoparticles has been the cornerstone of development of nanotechnology and its applications, as it emphasizes on promoting environmental sustainability. The aim of the present study was to investigate the potential health benefits of the green-synthesized titanium nanoparticles (TiO2NPs). TiO2NPs were synthesized using titanium(iv) isopropoxide and lupin bean extract. The synthesized particles were characterized to assess the average particle size by dynamic light scattering, and X-ray diffraction method was used to study the crystalline nature. The average particle size recorded was 9.227 nm with a polydispersity index (PDI) of 0.382. The morphology of the particles was assessed by scanning electron microscope and transmission electron microscopy which showed varied shapes of the nanoparticles, uniform spherical and crystallite rod shaped. Further, the cytotoxic efficacy of the nanoparticles was assessed against the breast cancer (MCF-7) cell line using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidefor (MTT) assay. The antibacterial activity of the nanoparticles was evaluated against clinical pathogens via the disc diffusion assay. The key findings showed that the TiO2NPs exhibited potent cytotoxicity against the MCF-7 cell line with an IC50 of 41.1 µg. It also showed profound antibacterial activity. Thus, the synthesized nanoparticles could have potential biomedical applications owing to their therapeutic efficacy.

中文翻译：

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钛纳米粒子的生态合成与表征：测试其细胞毒性和抗菌作用

摘要 近年来，纳米粒子的绿色合成一直是纳米技术及其应用发展的基石，因为它强调促进环境的可持续性。本研究的目的是调查绿色合成的钛纳米粒子 (TiO2NPs) 的潜在健康益处。使用异丙醇钛 (iv) 和羽扇豆提取物合成了 TiO2NP。通过动态光散射表征合成的颗粒以评估平均粒径，并使用X射线衍射方法研究结晶性质。记录的平均粒径为 9.227 nm，多分散指数 (PDI) 为 0.382。通过扫描电子显微镜和透射电子显微镜评估颗粒的形态，显示出不同形状的纳米颗粒，均匀的球形和微晶棒状。此外，使用溴化 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑鎓 (MTT) 测定法评估纳米颗粒对乳腺癌 (MCF-7) 细胞系的细胞毒性功效。通过圆盘扩散试验评估纳米颗粒对临床病原体的抗菌活性。主要发现表明，TiO2NPs 对 MCF-7 细胞系显示出有效的细胞毒性，IC50 为 41.1 µg。它还显示出强大的抗菌活性。因此，合成的纳米粒子由于其治疗功效而具有潜在的生物医学应用。5-二苯基溴化四唑（MTT）测定。通过圆盘扩散试验评估纳米颗粒对临床病原体的抗菌活性。主要发现表明，TiO2NPs 对 MCF-7 细胞系显示出有效的细胞毒性，IC50 为 41.1 µg。它还显示出强大的抗菌活性。因此，合成的纳米粒子由于其治疗功效而具有潜在的生物医学应用。5-二苯基溴化四唑（MTT）测定。通过圆盘扩散试验评估纳米颗粒对临床病原体的抗菌活性。主要发现表明，TiO2NPs 对 MCF-7 细胞系显示出有效的细胞毒性，IC50 为 41.1 µg。它还显示出强大的抗菌活性。因此，合成的纳米粒子由于其治疗功效而具有潜在的生物医学应用。