Abstract

Plantamajoside (PMS) is a major compound of Plantago asiatica and possesses anti-tumor property in several types of cancers. However, the effect of PMS on cervical cancer has not been investigated. This study aimed to investigate the effect of PMS on the migration and invasion of cervical cancer cell lines under hypoxic condition. Our results demonstrated that PMS significantly inhibited hypoxia-caused increases in cell migration and invasion of cervical cancer cells. The hypoxia-induced epithelial-mesenchymal transition (EMT) process was prevented by PMS with increased E-cadherin expression, and decreased expression levels of N-cadherin and vimentin in cervical cancer cells. Besides, the expression levels of transcription factors slug and snail were suppressed by PMS in hypoxia-induced cervical cancer cells. The increased mRNA and protein levels of hypoxia-inducible factor 1alpha (HIF-1α) in hypoxia-induced cervical cancer cells were prevented by PMS. Furthermore, PMS blocked the hypoxia-induced activation of NF-κB and PI3K/Akt pathway in cervical cancer cells. Taken together, these findings suggest that PMS exerted an anti-tumor activity in cervical cancer through preventing the hypoxia-induced EMT. Thus, PMS might serve as a therapeutic agent for the treatment of cervical cancer.

摘要

车前草苷 (PMS) 是车前子的主要化合物，在多种癌症中具有抗肿瘤特性。然而，PMS对宫颈癌的影响尚未得到研究。本研究旨在探讨PMS对缺氧条件下宫颈癌细胞系迁移和侵袭的影响。我们的结果表明，经前综合症显着抑制了缺氧引起的宫颈癌细胞迁移和侵袭的增加。缺氧诱导的上皮间质转化 (EMT) 过程被 PMS 阻止，E-钙粘蛋白表达增加，宫颈癌细胞中 N-钙粘蛋白和波形蛋白的表达水平降低。此外，PMS 抑制了缺氧诱导的宫颈癌细胞中转录因子 slug 和 snail 的表达水平。PMS阻止了缺氧诱导的宫颈癌细胞中缺氧诱导因子1α（HIF-1α）的mRNA和蛋白质水平的增加。此外，经前综合症阻断了宫颈癌细胞中缺氧诱导的 NF-κB 和 PI3K/Akt 通路的激活。总之，这些发现表明 PMS 通过阻止缺氧诱导的 EMT 在宫颈癌中发挥抗肿瘤活性。因此，经前综合症可作为治疗宫颈癌的治疗剂。