ABSTRACT Lily is one of the most valuable fresh cut flowers in the world. However, pollen pollution brings inconvenience to retailers and consumers. Anther development is a complicated and precise process but much is unknown. In this study, the anther development key gene LoAMS was isolated from Lilium Oriental Hybrids cultivar ‘Siberia’. Phylogenetic tree analysis and multiple sequence alignment showed that LoAMS protein had a typical basic helix–loop–helix domain (bHLH), belonging to the MYC transcription factor family. Furthermore, the subcellular localization experiment revealed that LoAMS was a nucleus protein. Transcriptional activation assays showed LoAMS was a transcriptional activator with the transcriptional activation domain in the last 204 amino acids (338-541aa) of the C-terminal region. The real-time quantitative PCR showed that LoAMS was abundant in lily anthers. Two peak expressions of LoAMS appeared in the anthers of 3-cm and 10-cm flower buds, especially in the microspores of 10-cm flower buds. These data indicate LoAMS is an anther specific transcriptional activator that may contribute to lily molecular breeding in the near future.

中文翻译：

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百合（东方百合）花药发育中LoAMS基因的分离与鉴定

摘要百合是世界上最有价值的鲜切花之一。然而，花粉污染给零售商和消费者带来了不便。花药发育是一个复杂而精确的过程，但还有很多未知。本研究从东方百合品种'西伯利亚'中分离得到花药发育关键基因LoAMS。系统发育树分析和多序列比对表明，LoAMS 蛋白具有典型的基本螺旋-环-螺旋结构域（bHLH），属于 MYC 转录因子家族。此外，亚细胞定位实验表明 LoAMS 是一种细胞核蛋白。转录激活分析表明 LoAMS 是一种转录激活因子，在 C 端区域的最后 204 个氨基酸 (338-541aa) 中具有转录激活域。实时定量 PCR 表明 LoAMS 在百合花药中含量丰富。LoAMS在3-cm和10-cm花蕾的花药中出现了两个高峰表达，尤其是在10-cm花蕾的小孢子中。这些数据表明 LoAMS 是一种花药特异性转录激活剂，在不久的将来可能有助于百合分子育种。