In this work, a β-cyclodextrin functional vinyl monomer was synthesized and the common moiety of five amanita toxins was used as the template for preparing molecularly imprinted polymers (MIPs). Chemical calculation was used to evaluate and describe the binding interactions between the template and the functional monomer. The preparation conditions were optimized and the resultant MIPs were characterized and employed as solid-phase extraction (SPE) sorbents. The SPE conditions including the amount of sorbent, extraction solution, and eluting solution were also optimized for the enrichment of the five toxins. Using an ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS), detection limits ranging from 0.34–0.42 µg/L, 0.16–0.33 µg/L, and 0.035–0.056 µg/kg were achieved for the five toxins in serum, urine and liver samples, respectively. The proposed method was further applied to the determination of the amanita toxins in suspected samples and showed great potential in the diagnosis of mushroom poisoning.

在这项工作中，合成了β-环糊精官能的乙烯基单体，并将五种鹅膏菌毒素的共同部分用作制备分子印迹聚合物（MIP）的模板。化学计算用于评估和描述模板与功能单体之间的结合相互作用。优化了制备条件，并对得到的MIP进行了表征并用作固相萃取（SPE）吸附剂。还优化了SPE条件，包括吸附剂，萃取溶液和洗脱溶液的量，以富集五种毒素。使用超高效液相色谱串联质谱法（UHPLC-MS / MS），对五种毒素的检出限分别为0.34–0.42 µg / L，0.16–0.33 µg / L和0.035–0.056 µg / kg在血清中 分别为尿液和肝脏样品。所提出的方法进一步应用于可疑样品中鹅膏菌毒素的测定，在蘑菇中毒的诊断中具有很大的潜力。