This study reports a preparation of silver nanoparticles (SNPs) using Microsorum pteropus methanol extract, as a new approach in the development of therapeutic strategies against diseases caused by oxidative stress, reactive oxygen, and nitrogen species. During the effort of extraction and isolation from M. pteropus, X-ray single-crystal structural analysis of sucrose was succeeded. 1,1-Diphenyl-2-picrylhydrazyl (DPPH) and hydrogen peroxide scavenging assay were used to confirm the antioxidant potential. Preparation of SNPs was confirmed by ultraviolet–visible (UV–Vis) spectra with peaks between 431 and 436 nm. Infrared (IR) analysis showed OH, NH functional groups of alcohol, phenol, amine, and aliphatic CH stretching vibrations of hydrocarbon chains of the synthesized nanoparticles. The antioxidant properties of the SNPs significantly showed DPPH reduction with an IC₅₀ value of 47.0 µg/mL and hydrogen peroxide scavenging activity with an IC₅₀ value of 35.8 µg/mL, and hence, indicating their capability to eliminate potentially damaging oxidants involved in oxidative stress and their related diseases.

这项研究报告了使用Microsorum pteropus甲醇提取物制备银纳米颗粒（SNP），作为开发针对由氧化应激，活性氧和氮物种引起的疾病的治疗策略的新方法。在从蕨类植物中提取和分离的过程中，成功进行了蔗糖的X射线单晶结构分析。1,1-二苯基-2-甲基苯并肼（DPPH）和过氧化氢清除法被用来确定抗氧化剂的潜力。SNP的制备已通过紫外可见（UV-Vis）光谱确认，其峰在431和436 nm之间。红外（IR）分析显示醇，酚，胺和脂肪族C的O H，N H官能团H拉伸合成的纳米颗粒的烃链的振动。SNP的抗氧化性能显着显示DPPH降低，IC₅₀值为47.0 µg / mL，过氧化氢清除活性为IC₅₀值为35.8 µg / mL，因此，表明它们具有消除潜在的破坏性氧化剂的能力，这些氧化剂涉及氧化应激和氧化应激。他们的相关疾病。