Treatments with follicle stimulating hormone (FSH) to enhance ovarian follicular development before ovum pick-up (OPU) are important for improving in-vitro embryo production (IVEP) procedures in cows, however, their procedural efficacy needs to be evaluated. The objective of the present study was to use meta-analytic methods to determine the effects of FSH treatments prior to OPU when there is progestin-priming on ovarian functions and IVEP in parous Bos taurus cows (n = 243). The literature was systematically reviewed and data from eight experiments, with 23 treatment means including 448 OPU sessions, were used for analyses. All eight experiments included a group of cows in which there was no FSH treatment (CON), and treatment with FSH before OPU to conduct IVEP. Mixed models were fitted using MIXED and GLIMMIX procedures of SAS. Additionally, classical meta-analytical statistics were also fitted using METAN and METAREG procedures of STATA. Models included fixed effects of treatment (i.e., CON and FSH) and random effect of the experiment, and data were weighted using the inverse of the SEM squared to account for precision of each experiment. Number of medium sized follicles, quality of oocytes, and transferable embryos were greater (P < 0.05) in FSH-treated as compared with cows of the CON group, without indications of publication bias or heterogeneity of data. Taken together, the results from the present study lead to the recommendation for treatments with FSH prior to OPU for IVEP when there was progestin-priming imposed to obtain a large number of transferable embryos for economic sustainability of commercial embryo transfer programs in parous non-lactating Bos taurus cows.

在取卵前用促卵泡激素 (FSH) 促进卵泡发育 (OPU) 对改善奶牛的体外胚胎生产 (IVEP) 程序很重要，但是，需要评估它们的程序有效性。本研究的目的是使用荟萃分析方法确定当孕激素启动对经产Bos taurus奶牛的卵巢功能和 IVEP 进行 OPU 之前 FSH 治疗的效果（n =243)。系统地回顾了文献，并使用来自 8 个实验的数据进行分析，其中包括 448 个 OPU 会话在内的 23 种治疗方法。所有八个实验都包括一组没有 FSH 治疗 (CON) 的奶牛，以及在 OPU 之前用 FSH 治疗进行 IVEP。混合模型使用 SAS 的 MIXED 和 GLIMMIX 程序拟合。此外，还使用 ​​STATA 的 METAN 和 METAREG 程序拟合了经典的元分析统计数据。模型包括治疗的固定效应（即 CON 和 FSH）和实验的随机效应，并且使用 SEM 平方的倒数对数据进行加权，以说明每个实验的精度。中等大小卵泡数、卵母细胞质量和可移植胚胎较多（P <0.05) 在 FSH 治疗中与 CON 组奶牛相比，没有发表偏倚或数据异质性的迹象。综上所述，本研究的结果导致建议在 OPU 之前对 IVEP 进行 FSH 治疗，当时为了获得大量可移植胚胎，在经产非哺乳期商业胚胎移植计划的经济可持续性Bos 金牛座奶牛。