Objective. Neural crest stem cells (NCSCs) are prototypically migratory cells immigrating from the dorsal neural tube to specific embryonic sites where they generate a variety of cell types. A lot of biomarkers for NCSCs have been identified. However, which biomarkers are the most specific is still unclear. Methods. The rat embryos harvested in embryonic day 9 (E9), E9.5, E10, E10.5, E11, E12, E13, and E14 were paraffin-embedded and sectioned in transverse. NCSCs were spatiotemporally demonstrated by immunohistochemical staining with RET, p75NTR, Pax7, and Sox10. NCSCs were isolated, cultured, and stained with RET, p75NTR, Pax7, and Sox10. Results. In the paraffin sections of rat embryos, the immunohistochemical staining of RET, p75NTR, and Sox10 can all be used in demonstrating NCSCs. Sox10 was positive mainly in NCSCs while RET and p75NTR were positive not only in NCSCs but also in other tissue cells. In primary culture cells, Sox10 was mainly in the nucleus of NCSCs, RET was mainly in the membrane, and p75NTR was positive in cytoplasm and membrane. Conclusions. Sox10 is the specific marker for immunohistochemical staining of NCSCs in paraffin sections. In cultured cells, Sox10, p75NTR, and RET presented a similar staining effect.

中文翻译：

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Sox10 是 Wistar 大鼠免疫组织化学染色中神经嵴干细胞的特异性生物标志物。

客观。神经嵴干细胞 (NCSC) 是典型的迁移细胞，从背侧神经管迁移到特定的胚胎部位，在那里它们产生各种细胞类型。已经确定了许多 NCSCs 的生物标志物。然而，哪些生物标志物是最具体的仍不清楚。方法。在胚胎第 9 天 (E9)、E9.5、E10、E10.5、E11、E12、E13 和 E14 收获的大鼠胚胎被石蜡包埋并横向切片。通过 RET、p75NTR、Pax7 和 Sox10 的免疫组织化学染色在时空上证明了 NCSC。NCSC 被分离、培养并用 RET、p75NTR、Pax7 和 Sox10 染色。结果. 在大鼠胚胎的石蜡切片中，RET、p75NTR 和 Sox10 的免疫组化染色均可用于展示 NCSC。Sox10 主要在 NCSCs 中呈阳性，而 RET 和 p75NTR 不仅在 NCSCs 中呈阳性，而且在其他组织细胞中也呈阳性。在原代培养细胞中，Sox10主要存在于NCSCs的细胞核中，RET主要存在于细胞膜中，p75NTR在细胞质和细胞膜中呈阳性。结论。Sox10 是石蜡切片中 NCSCs 免疫组化染色的特异性标志物。在培养的细胞中，Sox10、p75NTR 和 RET 呈现出相似的染色效果。