This study aimed to optimize a new sample preparation method using N-doped mesoporous carbon sorbent for simultaneous measurement of 1-naphthol and 2-naphthol, the biomarkers of exposure to naphthalene. The samples were analyzed using high-performance liquid chromatography supplied with ultraviolet detector (HPLC-UV). N-doped mesoporous carbon sorbent was obtained via the hard template procedure. The synthesized nanosorbent was then characterized by transmission electron microscopy (TEM), Brunauer-Emmett-Teller (BET), and elemental analysis (CHN). The effective factors in the extraction of the studied biomarkers were examined by the Box-Behnken (BBD) methodology. Regarding the optimum conditions, the sketched calibration curve for naphthols was linear in the concentration levels of 1–600 µg L^-1 for human urine samples. The accuracy and reproducibility of the introduced method were determined using the relative recovery (RR %) and relative standard deviation (RSD %) tests on the fortified urine samples. RR% and RSD% were found to be 97.0–101.2% and 3.1–9.0%, respectively. The calculated method detection limit of the optimized procedure was 0.3 µg L^-1 and 0.5 µg L^-1 for 1-naphthol and 2-naphthol, respectively.

这项研究旨在优化一种新的样品制备方法，该方法使用N掺杂的中孔碳吸附剂同时测量1-萘和2-萘酚（萘的生物标志物）。使用配有紫外检测器（HPLC-UV）的高效液相色谱法分析样品。通过硬模板程序获得N掺杂的中孔碳吸附剂。然后通过透射电子显微镜（TEM），Brunauer-Emmett-Teller（BET）和元素分析（CHN）对合成的纳米吸附剂进行表征。通过Box-Behnken（BBD）方法检查了提取研究的生物标志物的有效因素。关于最佳条件，绘制的萘酚校准曲线在1–600 µg L ^-1的浓度水平上是线性的用于人类尿液样本。使用强化尿液样品的相对回收率（RR％）和相对标准偏差（RSD％）测试确定引入方法的准确性和可重复性。发现RR％和RSD％分别为97.0–101.2％和3.1–9.0％。对于1-萘酚和2-萘酚，计算得出的最佳方法的方法检出限分别为0.3 µg L ^-1和0.5 µg L ^-1。