Cardamine violifolia (CV) is an important raw material for selenoprotein production. In this study, the protein of CV was extracted using conventional extraction (CE), ultrasound assisted extraction (UAE), pulsed electric pretreatment followed by CE (PEF + CE), and pulsed electric pretreatment followed by UAE (PEF + UAE), respectively. The result indicated that the yields of protein varied with extraction temperatures from 30 to 70 °C, while the highest yield was obtained at 40 °C for all these four methods. UAE showed the most effective extraction with a higher purity of protein powder (77%) and total selenium content (9097.33 ± 35.66 mg/kg). By application of response surface methodology, the protein yield was enhanced to 57.35 ± 0.92% at ultrasonic duration of 27 min, extraction temperature of 37 °C, and ultrasonic energy density of 8.125 W/mL. The obtained protein was further hydrolyzed by alcalase (37 °C, pH 10 for 5 h) and separated through ultrafiltration and size exclusion chromatography. Due to gel permeation chromatography-multi angle laser light scattering (GPC-MALLS) analysis, it was found that the CV protein hydrolysates were rich in dipeptides and tripeptides indicating promising antioxidant activities. Therefore, this study will contribute to the fabrication of selenium-rich proteins and antioxidant peptides as functional food ingredients.

豆蔻小叶（CV）是生产硒蛋白的重要原料。在这项研究中，分别使用常规提取（CE），超声辅助提取（UAE），脉冲电预处理和CE（PEF + CE）以及脉冲电预处理和UAE（PEF + UAE）提取CV蛋白质。结果表明，蛋白质的得率随提取温度从30到70°C而变化，而这四种方法在40°C时都获得了最高的得率。阿联酋显示出最有效的提取方法，蛋白质粉纯度更高（77％），总硒含量（9097.33±35.66 mg / kg）。通过应用响应面方法，在27分钟的超声持续时间，37°C的提取温度和8.125 W / mL的超声能量密度下，蛋白质产率提高到57.35±0.92％。将获得的蛋白质进一步用碱性蛋白酶水解（37°C，pH 10，持续5 h），并通过超滤和尺寸排阻色谱分离。由于凝胶渗透色谱-多角度激光散射（GPC-MALLS）分析，发现CV蛋白水解产物富含二肽和三肽，表明有希望的抗氧化剂活性。因此，这项研究将有助于制造富硒的蛋白质和抗氧化剂肽作为功能性食品成分。发现CV蛋白水解物富含二肽和三肽，表明有希望的抗氧化活性。因此，这项研究将有助于制造富硒的蛋白质和抗氧化剂肽作为功能性食品成分。发现CV蛋白水解物富含二肽和三肽，表明有希望的抗氧化活性。因此，这项研究将有助于制造富硒的蛋白质和抗氧化剂肽作为功能性食品成分。