Soils represent the natural habitat of entomopathogenic nematodes (EPNs). When moving in soil, EPNs are oriented to follow a chemical signal (chemotaxis). Cannabis sativa L. is known to secrete a very wide spectrum of secondary metabolites. Ethanol extracts (EE) of different C. sativa L. organs were used to study the effect on EPN chemotaxis. The root, leaf and inflorescence EE of two hemp varieties and two medical cannabis breeding lines were used in laboratory chemotaxis assays with three EPN species (Steinernema carpocapsae, S. feltiae and Heterorhabditis bacteriophora) at 20 and 25 °C. The content of terpenes and cannabinoids in C. sativa L. inflorescences was measured as well. Overall, EPNs were most attracted or repelled by inflorescence extracts, followed by leaves and roots. The most abundant terpene in hemp inflorescences was trans caryophyllene (38.2% on average), the highest contents of total cannabidiol, total cannabigerol and total tetrahydrocannabinol were 9.65%, 0.89% and 0.62%, respectively. The attraction effect of S. sativa L. EE is a highly interesting outcome and could lead to the development of attractant compounds in EPN biological control. Cannabinoids and terpenes may be responsible for pronounced effects on EPN chemotaxis.

中文翻译：

---

大麻根、叶和花序乙醇提取物对昆虫病原线虫化学营养反应的影响

土壤代表昆虫病原线虫 (EPN) 的自然栖息地。在土壤中移动时，EPN 的方向是遵循化学信号（趋化性）。已知 Cannabis sativa L. 会分泌非常广泛的次级代谢物。不同 C. sativa L. 器官的乙醇提取物 (EE) 用于研究对 EPN 趋化性的影响。两个大麻品种和两个医用大麻育种系的根、叶和花序 EE 在 20 和 25°C 下用于实验室趋化性测定，其中包括三种 EPN 物种（Steinernema carpocapsae、S. Feeliae 和 Heterorhabditis bacteriophora）。还测量了 C. sativa L. 花序中萜烯和大麻素的含量。总体而言，EPN 最受花序提取物的吸引或排斥，其次是叶和根。大麻花序中含量最多的萜烯是反式石竹烯（平均38.2%），总大麻二酚、总大麻酚和总四氢大麻酚含量最高，分别为9.65%、0.89%和0.62%。S. sativa L. EE 的吸引效应是一个非常有趣的结果，可能导致在 EPN 生物控制中开发引诱剂化合物。大麻素和萜烯可能对 EPN 趋化性产生显着影响。