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ANEUPLOIDY DURING THE ONSET OF MOUSE EMBRYO DEVELOPMENT
Reproduction ( IF 3.8 ) Pub Date : 2020-11-01 , DOI: 10.1530/rep-20-0086
Tereza Pauerova 1, 2 , Lenka Radonova 1, 2 , Kristina Kovacovicova 1 , Lucia Novakova 1, 2 , Michal Skultety 1, 2 , Martin Anger 1, 2
Affiliation  

Aneuploidy is the most frequent single cause leading into the termination of early development in human and animal reproduction. Although the mouse is frequently used as a model organism for studying the aneuploidy, we have only incomplete information about the frequency of numerical chromosomal aberrations throughout development, usually limited to a particular stage or assumed from the occurrence of micronuclei. In our study, we systematically scored aneuploidy in in vivo mouse embryos, from zygotes up to 16-cell stage, using kinetochore counting assay. We show here that the frequency of aneuploidy per blastomere remains relatively similar from zygotes until 8-cell embryos and then increases in 16-cell embryos. Due to the accumulation of blastomeres, aneuploidy per embryo increases gradually during this developmental period. Our data also revealed that the aneuploidy from zygotes and 2-cell embryos does not propagate further into later developmental stages, suggesting that embryos suffering from aneuploidy are eliminated at this stage. Experiments with reconstituted live embryos revealed, that hyperploid blastomeres survive early development, although they exhibit slower cell cycle progression and suffer frequently from DNA fragmentation and cell cycle arrest.

中文翻译:

小鼠胚胎发育开始期间的非整倍体

非整倍体是导致人类和动物繁殖早期发育终止的最常见的单一原因。虽然小鼠经常被用作研究非整倍体的模式生物,但我们只有关于整个发育过程中数值染色体畸变频率的不完整信息,通常仅限于特定阶段或假设来自微核的发生。在我们的研究中,我们使用动粒计数测定系统地对体内小鼠胚胎的非整倍性进行了评分,从受精卵到 16 细胞阶段。我们在这里展示了每个卵裂球的非整倍体频率从受精卵到 8 细胞胚胎保持相对相似,然后在 16 细胞胚胎中增加。由于卵裂球的积累,每个胚胎的非整倍体在这个发育时期逐渐增加。我们的数据还显示,受精卵和 2 细胞胚胎的非整倍性不会进一步传播到后期发育阶段,这表明患有非整倍性的胚胎在这个阶段被消除。重组活胚胎的实验表明,超倍体卵裂球在早期发育中存活下来,尽管它们表现出较慢的细胞周期进程并且经常遭受 DNA 断裂和细胞周期停滞。
更新日期:2020-11-01
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