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Digestive gland microbiome of Pleurobema cordatum: mesocosms induce dysbiosis
Journal of Molluscan Studies ( IF 1.2 ) Pub Date : 2020-08-28 , DOI: 10.1093/mollus/eyaa024
Alison K Aceves 1 , Paul D Johnson 2 , Carla L Atkinson 3 , Brian C van Ee 3 , Stephen A Bullard 1 , Cova R Arias 1
Affiliation  

ABSTRACT
Herein, we characterized the digestive gland (‘gut’) bacterial community (microbiome) of the Ohio pigtoe, Pleurobema cordatum (Rafinesque, 1820), using 16S rRNA gene sequencing. Two populations were compared: wild P. cordatum (n = 5) from the Tennessee River and P. cordatum (= 9) relocated to artificial mesocosms and exposed to various thermal regimes for 2 weeks. We also characterized the bacterial communities from the habitat (water and sediment) of these wild and mesocosm-held populations. The gut microbiome of wild P. cordatum was dominated by members of the bacterial phylum Tenericutes (72%). By contrast, the gut microbiome of mesocosm-held P. cordatum was dominated by members of the bacterial phylum Proteobacteria (64%). We found no temperature-associated difference in the gut microbiome of mesocosm-held P. cordatum. The bacterial communities of water and sediment from the Tennessee River were diverse and distinct from those of the studied mussels. By contrast, the bacterial communities of water and sediment in the mesocosms were dominated by Proteobacteria. These results suggest that when the studied mussels were moved into artificial rearing environments, their gut microbiome shifted to reflect that of their habitat (i.e. an increase in Proteobacteria). Moreover, the abundance of Tenericutes (also previously reported in other unionids) was reduced from 72% in wild mussels to 3% in mesocosm-held mussels. As a result, we think that mesocosm-held P. cordatum became dysbiotic, which could explain the observed wasting syndrome and associated trickling mortalities in captive P. cordatum.


中文翻译:

胸膜肺炎的消化腺微生物组:中胚层诱导营养不良

摘要
在本文中,我们使用16S rRNA基因测序来表征俄亥俄州小猪Pleurobema cordatum(Rafinesque,1820)的消化腺('gut')细菌群落(微生物组)。比较了两个种群: 来自田纳西河的野生P. cordatumn = 5)和重新安置到人工中观世界的P. cordatum = 9),并在各种热环境下暴露了2周。我们还从这些野生和中观持有的种群的栖息地(水和沉积物)中表征了细菌群落。野生P. cordatum的肠道微生物组主要由细菌门氏菌门类的成员(72%)主导。相比之下,中膜持有的脐带假单胞菌的肠道微生物组细菌门属Proteobacteria(64%)的成员占主导地位。我们发现在中膜保持的脐带假单胞菌的肠道微生物组中没有温度相关的差异。来自田纳西河的水和沉积物的细菌群落是多种多样的,并且与所研究的贻贝不同。相比之下,中膜中水和沉积物的细菌群落主要是变形杆菌。这些结果表明,当将所研究的贻贝移至人工饲养环境中时,其肠道微生物组会发生变化,以反映其栖息地的微生物(即,变形杆菌的增加)。此外,Tenericutes的丰度(先前也曾在其他工会中报道过)从野生贻贝的72%降低到中膜保存的贻贝的3%。结果,我们认为中观P. cordatum变成了不良生物,这可以解释圈养的P. cordatum中观察到的消瘦综合症和相关的ling流死亡率。
更新日期:2020-10-30
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