Lung adenocarcinoma (LUAD) is a highly malignant cancer. Although competing endogenous RNA (ceRNA)‐based profiling has been investigated in patients with LUAD, it has not been specifically used to study metastasis in LUAD. We found 130 differentially expressed (DE) lncRNAs, 32 DE miRNAs and 981 DE mRNAs from patients with LUAD in The Cancer Genome Atlas (TCGA) database. We analysed the functions and pathways of 981 DE mRNAs using the Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Based on the target DE mRNAs and DE lncRNAs of DE miRNAs, we established an lncRNA‐miRNA‐mRNA ceRNA network, comprising 37 DE lncRNAs, 22 DE miRNAs and 212 DE mRNAs. Subsequently, we constructed a protein‐protein interaction network of DE mRNAs in the ceRNA network. Among all, DE RNAs, 5 DE lncRNAs, 5 DE miRNAs and 45 DE mRNAs were confirmed found to be associated with clinical prognosis. Moreover, 3 DE lncRNAs, 4 DE miRNAs and 9 DE mRNAs in the ceRNA network were associated with clinical prognosis. We further screened 3 DE lncRNAs, 3 DE miRNAs and 3 DE mRNAs using clinical samples. These DE lncRNAs, DE miRNAs and DE mRNAs in ceRNA network may serve as independent biomarkers of LUAD metastasis.

中文翻译：

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转移性肺腺癌中非编码RNA相关的ceRNA网络的表征。

肺腺癌（LUAD）是高度恶性的癌症。尽管已经在LUAD患者中研究了基于竞争性内源RNA（ceRNA）的分析，但尚未专门用于研究LUAD的转移。我们在癌症基因组图谱（TCGA）数据库中发现了来自LUAD患者的130个差异表达（DE）lncRNA，32个DE miRNA和981 DE mRNA。我们使用基因本体论（GO）和《京都基因与基因组百科全书》（KEGG）数据库分析了981 DE mRNA的功能和途径。基于DE miRNA的目标DE mRNA和DE lncRNA，我们建立了一个lncRNA‐miRNA‐mRNA ceRNA网络，包括37个DE lncRNA，22个DE miRNA和212个DE mRNA。随后，我们在ceRNA网络中构建了DE mRNA的蛋白质-蛋白质相互作用网络。其中包括DE RNA，5个DE lncRNA，证实5个DE miRNA和45个DE mRNA与临床预后有关。此外，ceRNA网络中的3个DE lncRNA，4个DE miRNA和9个DE mRNA与临床预后相关。我们使用临床样品进一步筛选了3个DE lncRNA，3个DE miRNA和3个DE mRNA。ceRNA网络中的这些DE lncRNA，DE miRNA和DE mRNA可作为LUAD转移的独立生物标志物。