Biosilicate is a bioactive glass–ceramic used in medical and dental applications. This study evaluated novel reparative materials composed of pure tricalcium silicate (TCS), 30% zirconium oxide (ZrO₂) and 10 or 20% biosilicate, in comparison with Biodentine. Setting time was evaluated based on ISO 6876 standard, radiopacity by radiographic analysis, solubility by mass loss, and pH by using a pH meter. Cytotoxicity was evaluated by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide and NR assays. Alkaline phosphatase (ALP) activity and alizarin red were used to evaluate cell bioactivity. Antimicrobial activity was assessed on Enterococcus faecalis by the direct contact test. The data were submitted to analysis of variance (ANOVA)/Tukey; Bonferroni and Kruskal–Wallis, and Dunn tests (α = 0.05). The association of Biosilicate with TCS + ZrO₂ had appropriate setting time, radiopacity, and solubility, alkaline pH, and antimicrobial activity. TCS and Biodentine showed higher ALP activity in 14 days than the control (serum‐free medium). All cements produced mineralized nodules. In conclusion, Biosilicate + TCS ZrO₂ decreased the setting time and increased the radiopacity in comparison to TCS. Biosilicate + TCS ZrO2 presented lower solubility and higher radiopacity than Biodentine. In addition, these experimental cements promoted antimicrobial activity and mineralization nodules formation, suggesting their potential for clinical use.

中文翻译：

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修复性硅酸三钙-ZrO2-生物硅酸盐复合材料的开发与评价

生物硅酸盐是一种生物活性玻璃陶瓷，用于医疗和牙科应用。与 Biodentine 相比，本研究评估了由纯硅酸三钙 (TCS)、30% 氧化锆 (ZrO _{2 ) 和 10% 或 20% 生物硅酸盐组成的新型修复材料。}根据 ISO 6876 标准评估凝固时间，通过射线照相分析评估射线不透性，通过质量损失评估溶解度，并使用 pH 计评估 pH。通过 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑和 NR 测定评估细胞毒性。碱性磷酸酶 (ALP) 活性和茜素红用于评估细胞生物活性。对粪肠球菌进行抗菌活性评估通过直接接触测试。将数据提交给方差分析 (ANOVA)/Tukey；Bonferroni 和 Kruskal-Wallis 以及 Dunn 检验 ( α = 0.05)。生物硅酸盐与 TCS + ZrO ₂的结合具有适当的凝固时间、射线不透性和溶解度、碱性 pH 值和抗菌活性。TCS 和 Biodentine 在 14 天内显示出比对照（无血清培养基）更高的 ALP 活性。所有水泥都产生矿化结核。总之，生物硅酸盐 + TCS ZrO ₂与 TCS 相比，减少了凝固时间并增加了射线不透性。与 Biodentine 相比，生物硅酸盐 + TCS ZrO2 具有更低的溶解度和更高的射线不透性。此外，这些实验性水泥促进了抗菌活性和矿化结节的形成，表明它们具有临床应用的潜力。