The current study was designed as (2 × 3) factorial groups of 20 and 40 mg Zn/kg diet from each inorganic (zinc oxide), organic (Zn‐methionine) and nanoparticle (nano‐zinc oxide) sources supplemented to six Nile tilapia (O. niloticus) fingerling diets. The results showed both organic and nano‐Zn‐administered groups, especially at 40 mg/kg, exhibited significant improvements in body weight, total gain, feed conversion ratio and specific growth rate as compared to inorganic Zn‐supplemented groups. Nano‐Zn, at 20 and 40 mg/kg diet, increased haemoglobin, WBC counts, as well as lysozyme, IgM, and serum bactericidal test compared with other sources. Increasing Zn levels progressively increased serum and muscle Zn contents in nano‐Zn groups. Antioxidant capacity (TAC, SOD, and CAT) also increased in nano‐Zn‐supplemented fish at 40 mg/kg diet. Although the mRNA expression profile of growth hormone (GH) and insulin‐like growth factor 1 (IGF‐1) showed an up‐regulation in both organic and nano‐Zn‐supplemented fish, a dose‐dependent up‐regulation was observed for the expression of hepatic antioxidant (SOD and GPx) and immune (IL‐1β and TNF‐α) encoding genes in nano‐Zn‐administered groups alone. The highest resistance against Aeromonas hydrophila was observed in the nano‐Zn‐supplemented group at 40 mg/kg diet. Thus, it can be concluded that zinc nanoparticles are potential alternatives to conventional zinc sources in Nile tilapia.

中文翻译：

---

比较不同锌源和含量的饮食对罗非鱼，尼罗罗非鱼生长性能，免疫反应和抗氧化活性的影响

当前的研究被设计为（2×3）分解因子组，分别来自20种和40 mg锌/ kg饮食，分别来自无机物（氧化锌），有机物（锌蛋氨酸）和纳米颗粒（纳米锌氧化物），并补充了六个尼罗罗非鱼。 （尼罗罗非鱼）鱼种饮食。结果表明，与无机锌补充组相比，有机和纳米锌施用组（特别是在40 mg / kg时）在体重，总增重，饲料转化率和比生长速率方面均表现出显着改善。与其他来源相比，以20和40 mg / kg的日粮摄入的Nano-Zn可增加血红蛋白，WBC计数以及溶菌酶，IgM和血清杀菌试验。锌水平的增加会逐渐增加纳米锌组中血清和肌肉中锌的含量。日粮添加40 mg / kg的纳米锌补充鱼的抗氧化能力（TAC，SOD和CAT）也增加。尽管生长激素（GH）和胰岛素样生长因子1（IGF-1的mRNA表达谱）在有机和纳米锌补充鱼中均显示出上调，肝脏抗氧化剂（SOD和GPx）的表达和免疫（IL-1β和TNF-α）编码基因的表达均呈剂量依赖性上调仅在纳米锌给药组中。在饮食中添加40 mg / kg的纳米锌补充组中，对嗜水气单胞菌的抵抗力最高。因此，可以得出结论，锌纳米颗粒是尼罗罗非鱼中常规锌源的潜在替代品。