This study was carried out to investigate the effects of enzymatic hydrolysates from poultry by‐products (EHPB) as protein source on turbot (Scophthalmus maximus). For an eight‐week feeding trial, the control group was fed a normal feed (0 EHPB), and the experimental groups were given four experimental diets, containing 80, 168, 255 and 340 g/kg EHPB, respectively. Results showed that 80 g/kg EHPB had no effect on the growth performance, and obviously increased the serum complement C3, complement C4 and SOD levels; ≥168 g/kg EHPB significantly decreased the crude fat content and triglyceride level, and increased the BUN and MDA levels; ≥255 g/kg EHPB significantly decreased the weight gain, apparent digestibility (AD) of nutrients, complement C3, complement C4 and SOD levels. The hepatic proteome analysis indicated that EHPB increased 5'‐AMP‐activated protein kinase (AMPK) level and might affect fat accumulation by regulating relevant enzymes. 16S rRNA sequencing showed that EHPB influenced the microbial community structure (R = 0.13, ANOSIM p = .006) and significantly decreased the abundance of Vibrio, but 255 g/kg EHPB increased the abundances of Phyllobacterium, Sphingomonas and Delftia, indicating that the high level of EHPB may induce accumulation of harmful substances in the intestine. Consequently, excessive EHPB impeded the growth and health status of fish, and about 80 g/kg EHPB is suitable for turbot growth and suppression of potential pathogens.

中文翻译：

---

家禽副产物（EHPB）的酶水解产物作为鱼粉的替代来源对大菱t（Scophthalmus maximus）生长性能，肝蛋白质组和肠道菌群的影响

这项研究的目的是研究家禽副产物（EHPB）的酶水解产物作为蛋白质来源对大菱turbo（Scophthalmus maximus）。在为期八周的喂养试验中，对照组以普通饲料（0 EHPB）喂养，实验组给予四种实验饮食，分别包含80、168、255和340 g / kg EHPB。结果表明：80 g / kg EHPB对生长性能无影响，明显增加血清补体C3，补体C4和SOD水平。≥168g / kg EHPB显着降低了粗脂肪含量和甘油三酸酯水平，并增加了BUN和MDA水平；≥255g / kg EHPB显着降低了体重增加，营养素，补体C3，补体C4和SOD水平的表观消化率（AD）。肝蛋白质组分析表明，EHPB可增加5'-AMP激活的蛋白激酶（AMPK）水平，并可能通过调节相关酶来影响脂肪积累。[R  = 0.13，ANOSIM p  = 0.006）和显著降低的丰度弧菌，但255克/ kg的EHPB增加的丰度Phyllobacterium，鞘氨醇单和代尔夫特，表明EHPB的高电平可诱导在肠的有害物质积累。因此，过量的EHPB阻碍了鱼类的生长和健康状况，并且约80 g / kg EHPB适用于菱t生长和抑制潜在病原体。