Porcine Reproductive and Respiratory Syndrome virus (PRRSV) is one of the main component of the porcine respiratory disease complex (PRDC), which strongly impact the pig production. Although PRRSV is often considered as a primary infection that eases subsequent respiratory coinfections, the possibility that other PRDC components may facilitate PRRSV infection has been largely overlooked. The main cellular targets of PRRSV are respiratory macrophages among them alveolar macrophages (AM) and pulmonary intravascular macrophages (PIM). AM, contrarily to PIM, are directly exposed to the external respiratory environment, among them co-infectious agents. In order to explore the possibility of a co-infections impact on the capacity of respiratory macrophages to replicate PRRSV, we proceed to in vitro infection of AM and PIM sampled from animals presenting different sanitary status, and tested the presence in the respiratory tract of these animals of the most common porcine respiratory pathogens (PCV2, Actinobacillus pleuropneumoniae, Mycoplasma hyopneumoniae, Mycoplasma hyorhinis, Mycoplasma floculare, Pasteurella multocida, Bordetella bronchiseptica, Streptoccocus suis). In this exploratory study with a limited number of animals, no statistic differences were observed between AM and PIM susceptibility to in vitro PRRSV infection, nor between AM coming from animals presenting very contrasting respiratory coinfection loads.

猪繁殖与呼吸综合症病毒（PRRSV）是猪呼吸系统疾病综合症（PRDC）的主要成分之一，对猪的生产有很大影响。尽管PRRSV通常被认为是缓解随后的呼吸道合并感染的原发性感染，但其他PRDC成分可能促进PRRSV感染的可能性已被大大忽略。PRRSV的主要细胞靶标是呼吸道巨噬细胞，其中包括肺泡巨噬细胞（AM）和肺血管内巨噬细胞（PIM）。与PIM相反，AM直接暴露于外部呼吸环境，其中包括共感染因子。为了探讨共感染对呼吸道巨噬细胞复制PRRSV能力的影响，我们进行了体外研究从表现出不同卫生状况的动物身上采集AM和PIM感染，并测试这些动物的呼吸道中最常见的猪呼吸道病原体（PCV2，胸膜肺炎放线杆菌，猪肺炎支原体，猪肺炎支原体，支原体支原体，多杀巴斯德氏菌，博德特氏菌支气管，Streptoccocus猪链球菌）。在这项针对数量有限的动物的探索性研究中，未发现AM和PIM对体外PRRSV感染的易感性，以及来自表现出明显不同的呼吸道合并感染的动物的AM之间的统计学差异。