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Mitochondrial Protrusions in Neuronal Cells.
iScience ( IF 5.8 ) Pub Date : 2020-08-29 , DOI: 10.1016/j.isci.2020.101514
Pamela J Yao 1 , Erden Eren 1 , Ronald S Petralia 2 , Jeffrey W Gu 1 , Ya-Xian Wang 2 , Dimitrios Kapogiannis 1
Affiliation  

Mitochondrial function relies on multiple quality control mechanisms, including the release of mitochondrial vesicles. To investigate the ultrastructure and prevalence of mitochondrial membranous protrusions (and, by extension, vesicles) in neurons, we surveyed mitochondria in rat and planarian brains using transmission electron microscopy (EM). We observed that mitochondrial protrusions mostly extend from the outer membrane. Leveraging available 3D EM datasets of the brain, we further analyzed mitochondrial protrusions in neurons of mouse and Drosophila brains, identifying high-resolution spatial views of these protrusions. To assess whether the abundance of mitochondrial protrusions and mitochondria-derived vesicles respond to cellular stress, we examined neurons expressing fluorescently tagged mitochondrial markers using confocal microscopy with Airyscan and found increased numbers of mitochondrial protrusions and vesicles with mild stress. Future studies using improved spatial resolution with added temporal information may further define the functional implications of mitochondrial protrusions and vesicles in neurons.



中文翻译:

神经元细胞中的线粒体突起。

线粒体功能依赖于多种质量控制机制,包括线粒体囊泡的释放。为了研究神经元中线粒体膜突起(以及囊泡)的超微结构和普遍性,我们使用透射电子显微镜(EM)研究了大鼠和涡虫大脑中的线粒体。我们观察到线粒体突起大多从外膜延伸。利用可用的大脑 3D EM 数据集,我们进一步分析了小鼠和果蝇大脑神经元中的线粒体突起,识别了这些突起的高分辨率空间视图。为了评估丰富的线粒体突起和线粒体衍生的囊泡是否对细胞应激做出反应,我们使用 Airyscan 共聚焦显微镜检查了表达荧光标记线粒体标记的神经元,发现轻度应激下线粒体突起和囊泡的数量增加。未来的研究使用改进的空间分辨率和添加的时间信息可能会进一步定义神经元中线粒体突起和囊泡的功能含义。

更新日期:2020-09-14
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