Different factors, such as basic fibroblast growth factor (bFGF) and kit ligand (KL), are used in ovarian cortical culture to promote activation of primordial follicles. In the present study, the effects of bFGF and KL, alone and in combination, were evaluated on human follicular activation and growth during in-situ cortical culture. Slow frozen-thawed human ovarian cortical tissues (n = 6) were cultured in 4 different groups: 1) control (base medium), 2) KL (base medium; BM + 100 ng/ml KL), 3) bFGF (BM + 100 ng/ml bFGF) and 4) bFGF + KL (BM + 100 ng/ml KL + 100 ng/ml bFGF) for a week. The proportion of morphologically normal and degenerated follicles at different developmental stages, secreted hormonal levels and specific gene expressions were compared. Although the proportion of growing follicles was higher than primordial counterpart in all cultured groups, no significant differences were observed among the cultured groups. In all cultured groups, anti-Müllerian hormone (AMH), progesterone and estradiol hormones levels increased after 7 days of culture, however, this increase was only significant for estradiol in the bFGF + KL group. The expression of Ki67 gene indicated an increase in ovarian cell proliferation in the three experimental groups compared to the control group, however this increment was only significant for the bFGF + KL group. It can be concluded that KL and bFGF factors individually have no beneficial effects on in-situ follicular growth, but their combination positively influences steroidogenesis of granulosa cells without significantly increasing the number of growing follicles.

中文翻译：

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碱性成纤维细胞生长因子与试剂盒配体联合促进人卵巢皮质培养过程中颗粒细胞的增殖、活性和类固醇生成

不同的因子，如碱性成纤维细胞生长因子 (bFGF) 和试剂盒配体 (KL)，用于卵巢皮质培养以促进原始卵泡的激活。在本研究中，评估了 bFGF 和 KL 单独和组合对原位皮层培养过程中人卵泡激活和生长的影响。缓慢冻融的人​​卵巢皮质组织 (n = 6) 在 4 个不同的组中培养：1) 对照（基础培养基），2) KL（基础培养基；BM + 100 ng/ml KL），3) bFGF (BM + 100 ng/ml bFGF) 和 4) bFGF + KL（BM + 100 ng/ml KL + 100 ng/ml bFGF）一周。比较了不同发育阶段形态正常和退化卵泡的比例、分泌的激素水平和特定基因表达。尽管在所有培养组中生长卵泡的比例高于原始对应物，但在培养组之间没有观察到显着差异。在所有培养组中，抗苗勒管激素 (AMH)、孕酮和雌二醇激素水平在培养 7 天后增加，然而，这种增加仅对 bFGF + KL 组中的雌二醇显着。Ki67 基因的表达表明，与对照组相比，三个实验组的卵巢细胞增殖增加，但这种增加仅对 bFGF + KL 组显着。可以得出结论，KL 和 bFGF 因子单独对原位卵泡生长没有有益影响，