Photosynthetic process of common bean plants (Phaseolus vulgaris L.) was evaluated from short-term root flooding and recovery conditions. Common bean plants (BRS Expedito genotype) were grown in single plastic pots (1 L), containing soil as substrate. At the early reproductive stage (R1), distilled water was added up to 20 mm above the soil surface to flood the root system of plants for 1 day. The flooding was maintained by fitting a second pot without holes. After 1 day of flooding, the pots without holes were removed to drain water and recover the plants. Control plants were kept under normoxia. Chlorophyll a fluorescence transient, gas exchange, glycolate oxidase, antioxidative enzymes, reactive oxygen species (ROS) and lipid peroxidation were measured in leaves upon flooding (1 day) and recovery (1, 3 and 7 days) conditions. Root flooding (1 day) induced decrease (two-fold) in CO₂ assimilation rate and did not recover even after 7 days of normoxic conditions was re-established, besides limited transpiration rate and decreases in stomatal conductance. Moreover, the continuous light energy absorption by chlorophylls induced an increase in fluorescence and heat and impaired the connectivity between photosystems I and II, leading to ROS formation. The antioxidative enzyme system induced upon flooding and recovery conditions did not deal efficiently with ROS, which led to oxidative damage (lipid peroxidation) in leaves of common bean. Therefore, short-term root flooding impairs photosynthetic process recovery of common bean plants upon re-establishment of normoxic conditions.

从短期根淹和恢复条件对普通豆类植物（菜豆）的光合过程进行了评估。普通豆类植物（BRS Expedito基因型）种植在单个塑料盆中（1升），以土壤为基质。在繁殖初期（R1），将蒸馏水加至土壤表面上方20毫米处，以淹没植物的根系1天。通过安装没有孔的第二个锅来保持水浸。淹水1天后，将没有孔的盆移开以排水并恢复植物。对照植物保持常氧。叶绿素a在淹水（1天）和恢复（1、3和7天）条件下，测量叶片的荧光瞬态，气体交换，乙醇酸氧化酶，抗氧化酶，活性氧（ROS）和脂质过氧化。根淹（1天）导致CO ₂减少（两倍）同化率，并且即使在正常的缺氧情况下恢复7天后也没有恢复，除了蒸腾速率受到限制并且气孔导度降低。此外，叶绿素持续吸收光能引起荧光和热量的增加，并损害了光系统I和II之间的连通性，从而导致ROS的形成。在淹没和恢复条件下诱导产生的抗氧化酶系统不能有效地处理ROS，从而导致普通豆叶片的氧化损伤（脂质过氧化）。因此，在恢复正常氧条件后，短期根系泛滥会损害普通豆类植物的光合作用恢复。