Journal of Nanoparticle Research ( IF 2.5 ) Pub Date : 2020-08-28 , DOI: 10.1007/s11051-020-04990-9 Niloofar Behdarvand , Maryam Bikhof Torbati , Masoud Shaabanzadeh
The strategy of drug encapsulation with biocompatible polymeric nanocapsules could be a promising approach to improve the delivery of poorly water-soluble drugs for cancer therapy. The aim of this study was to investigate the drug delivery of tamoxifen from biocompatible nanocapsules made of poly(D,L-lactide) (PLA) core and 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)-2000] (DPPE-PEG) shell in the treatment of breast cancer. The analytical techniques such as FTIR, XRD, SEM, and TEM were used to investigate the structure of nanocapsules. Tamoxifen drug encapsulation efficiency and loading percentages as well as release kinetics at different pH values and temperatures were used to confirm the appropriate performance of these novel nanocapsules. The maximum tamoxifen release occurred at 40 °C, pH = 4. Based on the SEM and TEM images, the sizes of nanocapsules were between 57 and 159 nm. In order to investigate the anticancer effects and cytotoxicity of encapsulated tamoxifen in PLA/DPPE-PEG nanocapsules, the tetrazolium-based colorimetric (MTT) assay and cell cycle arrest analysis on MCF-7 breast cancer cell were performed. Also, cell cycle–related gene expressions of p53 and p21 in treated MCF-7 cells with tamoxifen-loaded PLA/DPPE-PEG nanocapsules were evaluated. The results of MTT cell proliferation experiments exhibited an increase in the cytotoxicity of tamoxifen nanocapsules on MCF-7 cell line compared with free tamoxifen. The 1.2-fold increase in the G1 stage compared with the control group in the cell cycle arrest protocol as well as the notable overexpression of p53 and p21 genes suggested that these nanocapsules arrested cell cycle in MCF-7 cells.
中文翻译:
载有他莫昔芬的PLA / DPPE-PEG脂质聚合物纳米胶囊通过细胞周期阻滞抑制雌激素阳性人乳腺癌细胞的生长
生物相容性聚合物纳米胶囊的药物包封策略可能是改善水溶性差的药物用于癌症治疗的一种有前途的方法。这项研究的目的是研究他莫昔芬从聚(D,L-丙交酯)(PLA)核和1,2-二棕榈酰-sn-甘油-3-磷酸乙醇胺-N- [甲氧基(聚乙二醇] -2000](DPPE-PEG)壳用于治疗乳腺癌。FTIR,XRD,SEM和TEM等分析技术被用于研究纳米胶囊的结构。他莫昔芬药物的包封效率和载量百分比以及在不同pH值和温度下的释放动力学被用来确认这些新型纳米胶囊的适当性能。他莫昔芬的最大释放发生在40°C,pH = 4的情况下。基于SEM和TEM图像,纳米胶囊的尺寸在57至159nm之间。为了研究封装的他莫昔芬在PLA / DPPE-PEG纳米胶囊中的抗癌作用和细胞毒性,对MCF-7乳腺癌细胞进行了基于四唑鎓的比色(MTT)测定和细胞周期阻滞分析。此外,还评估了装有他莫昔芬的PLA / DPPE-PEG纳米胶囊处理的MCF-7细胞中与细胞周期相关的p53和p21基因表达。MTT细胞增殖实验的结果显示,与游离他莫昔芬相比,他莫昔芬纳米胶囊对MCF-7细胞系的细胞毒性增加。1。