The purpose of this study was to investigate the endogenous cathepsin activity in each subcellular fraction and the effect of this activity on myofibrillar protein and texture during refrigeration and partial freezing storage of northern pike (Esox lucius) fillets. The results showed that fillets stored under the refrigerated condition were more susceptible to oxidation than partial freezing. Endogenous cathepsin activity indicated that partial freezing destroys the integrity of lysosomes more effectively than refrigeration and inhibits the increase in cathepsin B and B + L in lysosomes. The activity of cathepsin B and B + L in lysosomes, mitochondria and myofibrils under the partial freezing conditions was always lower than that under refrigeration. Texture analysis showed that refrigeration had a negative impact on hardness and springiness. In conclusion, the cathepsin activity in each subcellular fraction was effectively inhibited and better textural characteristics were obtained with partial freezing than refrigeration.

本研究的目的是研究每个亚细胞部分的内源组织蛋白酶活性，以及​​这种活性对北梭鱼 ( Esox lucius)冷藏和部分冷冻储存过程中肌原纤维蛋白和质地的影响。) 鱼片。结果表明，冷藏条件下储存的鱼片比部分冷冻更容易氧化。内源性组织蛋白酶活性表明，部分冷冻比冷藏更有效地破坏了溶酶体的完整性，并抑制了溶酶体中组织蛋白酶 B 和 B + L 的增加。部分冷冻条件下溶酶体、线粒体和肌原纤维中组织蛋白酶B和B+L的活性始终低于冷藏条件。质地分析表明冷藏对硬度和弹性有负面影响。总之，与冷藏相比，部分冷冻可有效抑制每个亚细胞级分中的组织蛋白酶活性，并获得更好的质地特性。