当前位置: X-MOL 学术Indian J. Exp. Biol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Potential of dumpsite bacterial isolate in producing polyhydroxybutyrate under stress prone environment
Indian Journal of Experimental Biology ( IF 0.6 ) Pub Date : 2020-08-28
N Chandani, PB Mazumder, A Bhattacharjee

Different bacterial species from soil samples of waste dumpsites were isolated and screened for their ability to synthesize polyhydroxybutyrate (PHB). Out of 86 bacterial cultures isolated, 26 isolates showed the presence of lipid granules PHB. Based on high polymer production, 4 potential bacterial isolates were identified by morphological, physiological and 16S rDNA sequencing. Their nucleotide sequences were submitted in the GenBank database under accession numbers KT907042, KT907043, KT907045 and KT907047. As biomass production is analogous to PHB production, cultural parameters like carbon, nitrogen, pH and temperature were optimized for maximum production of polymer by a potential bacterium Bacillus pumilus K8. Growth kinetics of B. pumilus K8 was analyzed and found that it grows at a rate 0.112 gh-1L-1 of the production medium. It could achieve maximum production of 72.93% of PHB at the rate of 0.013 gg-1 of biomass h-1 with polymer concentration of 5.04 gL-1 using mannitol, yeast extract, pH 7 and temperature 35°C, respectively as favourable cultural parameters. phbC gene, mainly responsible for PHB production was amplified by PCR and this genotypically confirms PHB production by the bacterial isolates. Bacillus pumilus K8 may be exploited for further industrial production of biopolymer.

中文翻译:

易受环境影响的垃圾场细菌分离物在生产聚羟基丁酸酯中的潜力

从废物堆放场的土壤样品中分离出不同的细菌种类,并筛选其合成聚羟基丁酸酯(PHB)的能力。在分离出的86种细菌培养物中,有26种分离物显示脂质颗粒PHB的存在。基于高产聚合物,通过形态,生理和16S rDNA测序鉴定了4种潜在的细菌分离株。它们的核苷酸序列在GenBank数据库中以登录号KT907042,KT907043,KT907045和KT907047提交。由于生物质的生产类似于PHB的生产,因此优化了碳,氮,pH和温度等培养参数,以通过潜在的短杆菌芽孢杆菌K8最大限度地生产聚合物。短双歧杆菌的生长动力学分析K8,发现其以生产培养基的0.112gh -1 L -1的速率生长。以甘露醇,酵母提取物,pH 7和温度35°C作为有利的培养参数,以生物量h -1的0.013 gg -1的速率,聚合物浓度为5.04 gL -1的条件下,PHB的最大产量可达72.93%。。通过PCR扩增了主要负责PHB产生的phbC基因,并从基因上证实了细菌分离株的PHB产生。短小芽孢杆菌K8可用于生物聚合物的进一步工业生产。
更新日期:2020-08-28
down
wechat
bug