This experiment is conducted to evaluate the anti-inflammatory effect of Piper chaba roots. The in-vitro anti-inflammatory activity of Piper chaba was carried out by human red blood cell (HRBC) membrane stabilization method which includes heat-induced hemolysis and hypo tonicity- induced hemolysis and also by another method of egg albumin denaturation assay. Anti-inflammatory activity study of crude ethanolic extract was performed using heat induced membrane stabilization method, hypo-tonicity induced HRBC membrane stabilization method and egg albumin denaturation method. Crude ethanolic extracts of P. chaba showed promising in vitro anti-inflammatory activity in a concentration dependent manner. Using acetyl salicylic acid (ASA) as standard drug and was compared with ethanolic extract to determine anti-inflammatory activity. Heat induced anti-inflammatory test revealed that crude ethanolic extract of P. chaba (500 μg/ml) and positive control ASA(500 μg/ml) have 52.667% and 78% respectively, hypo tonicity induced anti-inflammatory test showed 35.67% and 59% inhibition of red blood cell (RBC) hemolysis. Egg albumin denaturation method also evaluated that crude ethanolic extract (1000 μg/ml) and ASA (1000 μg/ml) showed 60% and 97.12% inhibition of egg albumin denaturation. The plant of P. chaba of the genus Piper possesses promising anti-inflammatory activities.

中文翻译：

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膜稳定作为彩的乙醇根提取物的抗炎活性的机制（荜茶吧）

进行该实验以评估胡椒木根的抗炎作用。美洲白桦的体外抗炎活性是通过人红细胞（HRBC）膜稳定化方法进行的，该方法包括热诱导的溶血和低张性诱导的溶血，以及卵清蛋白变性测定的另一种方法。采用热诱导膜稳定化方法，低渗诱导性HRBC膜稳定化方法和卵清蛋白变性法进行了粗乙醇提取物的抗炎活性研究。车前假单胞菌的粗乙醇提取物以浓度依赖性的方式显示出有希望的体外抗炎活性。使用乙酰水杨酸（ASA）作为标准药物，并与乙醇提取物进行比较以确定抗炎活性。热诱导的抗炎试验表明，车前假单胞菌的粗乙醇提取物（500μg/ ml）和阳性对照ASA（500μg/ ml）分别占52.667％和78％，低渗性诱导的抗炎试验显示为35.67％和抑制59％的红细胞（RBC）溶血。鸡蛋清蛋白变性方法还评估了粗乙醇提取物（1000μg/ ml）和ASA（1000μg/ ml）对鸡蛋清蛋白变性的抑制作用分别为60％和97.12％。Piper属的cha.P.chaba植物具有有希望的抗炎活性。鸡蛋清蛋白变性方法还评估了粗乙醇提取物（1000μg/ ml）和ASA（1000μg/ ml）对鸡蛋清蛋白变性的抑制作用分别为60％和97.12％。Piper属的cha.P.chaba植物具有有希望的抗炎活性。鸡蛋清蛋白变性方法还评估了粗乙醇提取物（1000μg/ ml）和ASA（1000μg/ ml）对鸡蛋清蛋白变性的抑制作用分别为60％和97.12％。Piper属的cha.P.chaba植物具有有希望的抗炎活性。