Exosomes, a form of extracellular vesicle, are an important precursor in regenerative medicine. Microfluidic methods exist to capture these sub-micrometer sized objects from small quantities of sample, ideal for multiple diagnostic applications. To address the challenge of extraction from large volumes, we use the visual access offered by microfluidic techniques to probe the physical mechanisms behind a method which is compatible with future upscaling. The sound wave actuated nano-sieve uses resonant modes in a packed bed of microparticles to exert trapping forces on nanoparticles. Here, we examine the role of the microparticle size, demonstrating better performance from 15 μm particles than 7 μm particles. When applied to biological samples, we demonstrate for the first time that a packed bed of these larger particles is capable of capturing exosomes and liposomes, the captured particles being on average 20 to 40 times smaller than the pores within the trapped bed.

中文翻译：

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使用声波激活的纳米筛（SWANS）捕获和富集外来体。

外泌体是细胞外囊泡的一种形式，是再生医学的重要前体。存在微流体方法，可以从少量样品中捕获这些亚微米级的物体，非常适合多种诊断应用。为了解决从大体积提取的挑战，我们使用微流体技术提供的可视通道来探查与将来的升级兼容的方法背后的物理机制。声波驱动的纳米筛利用微粒填充床中的共振模式在纳米粒子上施加捕获力。在这里，我们检查了微粒尺寸的作用，证明了15μm微粒的性能优于7μm微粒。当应用于生物样品时，