Background. Increasing evidence has revealed that mesenchymal stromal cell (MSC) transplantation alleviates hypoxic-ischemic brain damage (HIBD) induced neurological impairments via immunomodulating astrocyte antiapoptosis effects. However, it remains unclear whether MSCs regulate neuron autophagy following HIBD. Results. In the present study, MSC transplantation effectively ameliorated learning-memory function and suppressed stress-induced hippocampal neuron autophagy in HIBD rats. Moreover, the suppressive effects of MSCs on autophagy were significantly weakened following endogenous IL-6 silencing in MSCs. Suppressing IL-6 expression also significantly increased p-AMPK protein expression and decreased p-mTOR protein expression in injured hippocampal neurons. Conclusion. Endogenous IL-6 in MSCs may reduce autophagy in hippocampal neurons partly through the AMPK/mTOR pathway.

中文翻译：

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间充质基质细胞抑制缺氧缺血性脑损伤诱导的海马神经元自噬应激：内源性 IL-6 分泌的可能作用。

背景。越来越多的证据表明，间充质基质细胞 (MSC) 移植通过免疫调节星形胶质细胞抗细胞凋亡作用减轻缺氧缺血性脑损伤 (HIBD) 诱导的神经损伤。然而，尚不清楚 MSCs 是否调节 HIBD 后的神经元自噬。结果。在本研究中，MSC 移植有效改善了 HIBD 大鼠的学习记忆功能并抑制了应激诱导的海马神经元自噬。此外，在 MSCs 中内源性 IL-6 沉默后，MSCs 对自噬的抑制作用显着减弱。抑制 IL-6 表达还显着增加了受损海马神经元中 p-AMPK 蛋白的表达并降低了 p-mTOR 蛋白的表达。结论. MSCs 中的内源性 IL-6 可能部分通过 AMPK/mTOR 通路减少海马神经元的自噬。