RWP-RK proteins are important factors involved in nitrate response and gametophyte development in plants, and the functions of RWP-RK proteins have been analyzed in many species. However, the characterization of peanut RWP-RK proteins is limited. In this study, we identified 16, 19, and 32 RWP-RK members from Arachis duranensis, Arachis ipaensis, and Arachis hypogaea, respectively, and investigated their evolution relationships. The RWP-RK proteins were classified into two groups, RWP-RK domain proteins and NODULE-INCEPTION-like proteins. Chromosomal distributions, gene structures, and conserved motifs of RWP-RK genes were compared among wild and cultivated peanuts. In addition, we identified 12 orthologous gene pairs from the two wild peanut species, 13 from A. duranensis and A. hypogaea, and 13 from A. ipaensis and A. hypogaea. One, one, and seventeen duplicated gene pairs were identified within the A. duranensis, A. ipaensis, and A. hypogaea genomes, respectively. Moreover, different numbers of cis-acting elements in the RWP-RK promoters were found in wild and cultivated species (87 in A. duranensis, 89 in A. ipaensis, and 92 in A. hypogaea), and as a result, many RWP-RK genes showed distinct expression patterns in different tissues. Our study will provide useful information for further functional and evolutionary analysis of the RWP-RK genes.

中文翻译：

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来自花生，花生和花生的RWP-RK蛋白的表征和比较分析。

RWP-RK蛋白是植物中硝酸盐反应和配子体发育的重要因素，并且已经在许多物种中分析了RWP-RK蛋白的功能。但是，花生RWP-RK蛋白的表征是有限的。在这项研究中，我们确定了16个，19个和32 RWP-RK从会员蔓花生，花生ipaensis，以及花生，分别与考察它们的进化关系。RWP-RK蛋白分为两类，RWP-RK结构域蛋白和结节样蛋白。RWP-RK的染色体分布，基因结构和保守基序比较了野生和栽培花生中的基因。此外，我们确定了从两个野生花生品种12直系同源基因对，从13 A. duranensis和A.桃林，以及来自13个A. ipaensis和A.桃林。其中，一，十七复制的基因对的内鉴定A. duranensis，A. ipaensis和A.桃林分别基因组。此外，不同数量的顺式作用中的元素RWP-RK启动子在野生发现和栽培种（87 A. duranensis，在89 A. ipaensis和92在A.。花生），因此许多RWP-RK基因在不同组织中表现出不同的表达模式。我们的研究将为RWP-RK基因的进一步功能和进化分析提供有用的信息。