Accumulating evidence suggests M2 macrophages contribute to tissue reparation and limit inflammation in multiple sclerosis (MS). However, most studies have focused on murine models without substantial support through human MS observations. The present study aimed to quantify the relative abundances of M2 macrophages in different lesion types excised from human MS patients. CIBERSORTx, an established RNA deconvolution algorithm, was applied on bulk RNA-sequencing data developed from 98 lesions from 10 progressive MS patients and 5 neuropathological control donors. A validated gene signature matrix for 22 human hematopoietic cell subsets was used to infer the relative proportions of immune cells that were present in the original lesion. Deconvolution of the bulk gene expression data showed that inactive lesions contained significantly more M2 macrophages compared to normal white matter control samples. The findings suggest that M2 macrophages may play a role during lesion inactivity in MS.

中文翻译：

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进行性多发性硬化转录组解卷积表明非活动病变中 M2 巨噬细胞增加

越来越多的证据表明 M2 巨噬细胞有助于组织修复并限制多发性硬化症 (MS) 的炎症。然而，大多数研究都集中在没有通过人类 MS 观察得到实质性支持的小鼠模型上。本研究旨在量化从人类 MS 患者身上切除的不同病变类型中 M2 巨噬细胞的相对丰度。CIBERSORTx 是一种成熟的 RNA 解卷积算法，应用于从 10 名进展性 MS 患者和 5 名神经病理学对照供体的 98 个病灶中开发的大量 RNA 测序数据。用于 22 个人类造血细胞亚群的经过验证的基因特征矩阵用于推断原始病变中存在的免疫细胞的相对比例。大量基因表达数据的解卷积显示，与正常白质对照样本相比，非活动病变包含显着更多的 M2 巨噬细胞。研究结果表明 M2 巨噬细胞可能在 MS 病变不活动期间发挥作用。