Hierarchically ordered scaffold has a great impact on cell patterning and tissue engineering. The introduction of controllable coils into a scaffold offers an additional unique structural feature compared to conventional linear patterned scaffolds and can greatly increase interior complexity and versatility. In this work, 3D coil compacted scaffolds with hierarchically ordered patterns and tunable coil densities created using speed-programmed melt electrospinning writing (sMEW) successfully led to in vitro cell growth in patterns with tunable cell density. Subcutaneous implantation in mice showed great in vivo biocompatibility, as evidenced by no significant increase in tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6) levels in mouse serum. In addition, a lumbar vertebra was successfully printed for mesenchymal stem cells to grow in the desired pattern. A long-range patterned matrix composed of programmable short-range compacted coils enabled the design of complex structures, e.g., for tailored implants, by readily depositing short-range coil-compacted secondary architectures along with customized primary design.

分层有序的支架对细胞模式和组织工程有很大的影响。与传统的线性图案化脚手架相比，将可控线圈引入脚手架提供了额外的独特结构特征，并且可以大大增加内部复杂性和多功能性。在这项工作中，使用速度编程的熔体静电纺丝书写（sMEW）创建的具有分层排序模式和可调线圈密度的3D线圈压实支架成功地导致了体外细胞在具有可调细胞密度的模式下生长。小鼠皮下植入显示出良好的体内生物相容性，如小鼠血清中的肿瘤坏死因子α（TNF-α）和白介素6（IL-6）水平没有明显增加所证明。此外，腰椎成功地打印，使间充质干细胞以所需的模式生长。由可编程短程压缩线圈组成的远程图案化矩阵，可以通过轻松沉积短程线圈紧凑的二级结构以及定制的主要设计，来设计复杂的结构，例如用于定制植入物。