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Efficient Detection and Single-Cell Extraction of Circulating Tumor Cells in Peripheral Blood
ACS Applied Bio Materials ( IF 4.7 ) Pub Date : 2020-08-28 , DOI: 10.1021/acsabm.0c00957
Rui Li 1, 2 , Zhiyi Gong 1 , Kezhen Yi 3 , Wei Li 1 , Yichao Liu 4 , Fubing Wang 3 , Shi-Shang Guo 1
Affiliation  

Circulating tumor cells (CTCs) play an important role in cancer biology studies. To further elucidate the role of single CTCs in tumor metastasis and prognosis, effective methods must be developed to isolate and encapsulate single CTCs. In this work, a single CTC capture and encapsulation platform based on ZnO nanofibers and surface acoustic waves was constructed. We also demonstrated that this platform can capture and encapsulate single CTCs efficiently. We first validated that the dense ZnO nanofibers provide additional binding sites, resulting in an increased capture efficiency of up to 93.3%. We then demonstrated that the release efficiency was increased to 100% by dissolving the substrate with ultralow concentration (25 mM) phosphoric acid, and the activity of the released cells was not affected. Furthermore, the released cells were suspended in alginate solution and encapsulated single CTCs via droplet-based surface acoustic wave devices. The encapsulating rate of a single cell is up to 13% under a pulse width duration of 520 μs. Few technology based on nanofibers and acoustic tweezers for single-cell encapsulation via acoustic droplet vaporization has been reported. It has a wide range of potential applications to acquire single target cells, which may facilitate further early clinical diagnosis and treatment of cancer patients.

中文翻译:

外周血循环肿瘤细胞的高效检测和单细胞提取

循环肿瘤细胞 (CTC) 在癌症生物学研究中发挥着重要作用。为了进一步阐明单个 CTC 在肿瘤转移和预后中的作用,必须开发有效的方法来分离和封装单个 CTC。在这项工作中,构建了基于 ZnO 纳米纤维和表面声波的单一 CTC 捕获和封装平台。我们还证明了该平台可以有效地捕获和封装单个 CTC。我们首先验证了致密的 ZnO 纳米纤维提供了额外的结合位点,从而提高了高达 93.3% 的捕获效率。然后我们证明,通过用超低浓度(25 mM)磷酸溶解底物,释放效率提高到 100%,并且释放细胞的活性不受影响。此外,释放的细胞悬浮在藻酸盐溶液中,并通过基于液滴的表面声波装置封装单个 CTC。在 520 μs 的脉宽持续时间下,单个电池的封装率高达 13%。很少有基于纳米纤维和声学镊子通过声学液滴蒸发进行单细胞封装的技术报道。它在获取单个靶细胞方面具有广泛的潜在应用,可能有助于癌症患者的进一步早期临床诊断和治疗。很少有基于纳米纤维和声学镊子通过声学液滴蒸发进行单细胞封装的技术报道。它在获取单个靶细胞方面具有广泛的潜在应用,可能有助于癌症患者的进一步早期临床诊断和治疗。很少有基于纳米纤维和声学镊子通过声学液滴蒸发进行单细胞封装的技术报道。它在获取单个靶细胞方面具有广泛的潜在应用,可能有助于癌症患者的进一步早期临床诊断和治疗。
更新日期:2020-09-21
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