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Diversity in the intrinsic apoptosis pathway of nematodes.
Communications Biology ( IF 5.9 ) Pub Date : 2020-08-28 , DOI: 10.1038/s42003-020-01208-5
Neil D Young 1 , Tiffany J Harris 2 , Marco Evangelista 2 , Sharon Tran 2, 3 , Merridee A Wouters 2, 4 , Tatiana P Soares da Costa 5 , Nadia J Kershaw 6 , Robin B Gasser 1 , Brian J Smith 5 , Erinna F Lee 2, 3, 5 , W Douglas Fairlie 2, 3, 5
Affiliation  

Early studies of the free-living nematode C. elegans informed us how BCL-2-regulated apoptosis in humans is regulated. However, subsequent studies showed C. elegans apoptosis has several unique features compared with human apoptosis. To date, there has been no detailed analysis of apoptosis regulators in nematodes other than C. elegans. Here, we discovered BCL-2 orthologues in 89 free-living and parasitic nematode taxa representing four evolutionary clades (I, III, IV and V). Unlike in C. elegans, 15 species possess multiple (two to five) BCL-2-like proteins, and some do not have any recognisable BCL-2 sequences. Functional studies provided no evidence that BAX/BAK proteins have evolved in nematodes, and structural studies of a BCL-2 protein from the basal clade I revealed it lacks a functionally important feature of the C. elegans orthologue. Clade I CED-4/APAF-1 proteins also possess WD40-repeat sequences associated with apoptosome assembly, not present in C. elegans, or other nematode taxa studied.



中文翻译:

线虫内在凋亡途径的多样性。

对自由生活线虫C. elegans 的早期研究告诉我们 BCL-2 调节的人类细胞凋亡是如何调节的。然而,随后的研究表明,与人类细胞凋亡相比,秀丽隐杆线虫的细胞凋亡具有几个独特的特征。迄今为止,除秀丽隐杆线虫外,还没有对线虫中的细胞凋亡调节因子进行详细分析。在这里,我们在代表四个进化进化枝(I、III、IV 和 V)的 89 个自由生活和寄生线虫类群中发现了 BCL-2 直向同源物。与C. elegans不同, 15 个物种拥有多种(2 到 5 个)BCL-2 样蛋白,有些没有任何可识别的 BCL-2 序列。功能研究没有提供 BAX/BAK 蛋白在线虫中进化的证据,对来自基底进化枝 I 的 BCL-2 蛋白的结构研究表明它缺乏秀丽隐杆线虫直向同源物的重要功能特征。进化枝 I CED-4/APAF-1 蛋白还具有与凋亡体组装相关的 WD40 重复序列,在秀丽隐杆线虫或其他研究的线虫分类群中不存在。

更新日期:2020-08-28
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