Abstract

The increased lead (Pb) pollution in the biosphere has resulted in serious environmental problems, so it is essential to evaluate phytoremediation strategies for contaminated soils. This study evaluated the growth and Pd absorption capacity of Pakinsonia aculeata, inoculated with an arbuscular mycorrhizal fungus (Rhizophagus intraradices) over 18 weeks under greenhouse conditions. Treatments included inoculated and non-inoculated plants combined with six Pb concentrations (0, 40, 80, 160, 320, 640 mg·L⁻¹) in the form of Pb(NO₃)₂. Results showed that mycorrhizal colonization in inoculated plants ranged from 5.0 to 6.7% and favored plant growth. Pb levels and AMF-inoculation had no effects on chlorophyll fluorescence values. AMF-plants absorbed significantly more Pb in roots (237.97 mg·kg⁻¹) than control plants (202.85 mg·kg⁻¹), as well as high translocation to shoots (27.02 mg·kg⁻¹) under the high Pb dose. The increase in Pb concentration reduced the P concentration in roots, and the P and N concentrations in shoots; however, the absorption and translocation of Ca and Mg was increased in shoots. Inoculation of R. intraradices improved both growth and Pb uptake of P. aculeata, under greenhouse conditions suggesting that this tree species may be potentially studied for detoxifying Pb-polluted soils.

摘要

生物圈中铅（Pb）污染的增加导致了严重的环境问题，因此，评估污染土壤的植物修复策略至关重要。这项研究评估了温室环境下在18周内接种丛枝菌根真菌（Rhizophagus intraradices）的Pakinsonia aculeata的生长和Pd吸收能力。处理包括接种的和未接种的植物，并以Pb（NO ₃）₂的形式结合六种Pb浓度（0、40、80、160、320、640 mg·L ^-1）。结果表明，接种植物的菌根定植率在5.0％至6.7％之间，有利于植物生长。铅水平和接种AMF对叶绿素荧光值没有影响。在高Pb剂量下，AMF植物在根部（237.97 mg·kg ^-1）的铅吸收量显着高于对照植物（202.85 mg·kg ^-1），并且在高Pb剂量下对茎的高易位性（27.02 mg·kg ^-1）。Pb浓度的增加会降低根中的P浓度，降低新梢中的P和N浓度。然而，芽中钙和镁的吸收和转运增加。接种R. intraradices改善的生长和铅吸收P. aculeata，在温室条件下，表明该树种可能被研究用于对Pb污染的土壤进行解毒。