Ganoderma neo-japonicum is a well-known medicinal mushroom in Asian countries. However, scientific validations on its curative activities are confined to cirrhosis and diabetes. In this study, the anticancer properties of G. neo-japonicum were evaluated using cellular and computational models. The ethanolic extract (EtOH) with a promising inhibitory effect was fractionated into four different fractions: hexane (Hex), chloroform (Chl), butanol (Btn), and aqueous (Aq). The active fractions were then subjected to cell apoptosis assessment and phytochemical profiling. Molecular docking was conducted to elucidate the affinity of selected constituents towards antiapoptotic Bcl-2 protein. The butanol fraction showed the highest antioxidant activities as well as total phenolic content. Both hexane and chloroform fractions exerted a potent cytotoxic effect on colonic carcinoma cells through the induction of apoptosis. Phytochemical analysis revealed that the chloroform fraction is terpenoid enriched whereas the hexane fraction comprises predominantly sterol constituents. Stellasterol and 1,25-dihydroxyvitamin D3 3-glycoside were demonstrated to have a high affinity towards Bcl-2 protein. Overall, G. neo-japonicum can be considered as a compelling therapeutic candidate for cancer treatment.

中文翻译：

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药用蘑菇 Ganoderma neo-japonicum Imazeki 对人结肠癌细胞的体外和计算机抗癌评价

新日本灵芝是亚洲国家著名的药用菌。然而，对其治疗活性的科学验证仅限于肝硬化和糖尿病。在这项研究中，G. neo-japonicum的抗癌特性使用细胞和计算模型进行评估。具有良好抑制作用的乙醇提取物 (EtOH) 被分成四个不同的部分：己烷 (Hex)、氯仿 (Chl)、丁醇 (Btn) 和水溶液 (Aq)。然后对活性部分进行细胞凋亡评估和植物化学分析。进行分子对接以阐明所选成分对抗凋亡 Bcl-2 蛋白的亲和力。丁醇部分显示出最高的抗氧化活性以及总酚含量。己烷和氯仿组分均通过诱导细胞凋亡对结肠癌细胞产生有效的细胞毒作用。植物化学分析表明，氯仿部分富含萜类化合物，而己烷部分主要包含甾醇成分。星甾醇和1，25-二羟基维生素 D3 3-糖苷被证明对 Bcl-2 蛋白具有高亲和力。总体，G. neo-japonicum可以被认为是一种引人注目的癌症治疗候选药物。