Cell–cell or cell–substrate interactions are lost when cells are dissociated in culture, or during pathophysiological breakdowns, therefore impairing their structure and polarity, and affecting their function. We show that single rat β‐cells, cultured under non‐adhesive conditions, form intracytoplasmic vacuoles increasing in number and size over time. We characterized these structures and their implication in β‐cell function.

中文翻译：

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单个胰腺β细胞中细胞-细胞和细胞-底物接触的丧失将胰岛素释放转移到细胞内囊泡区室

当细胞在培养中解离或病理生理崩溃时，细胞-细胞或细胞-底物相互作用会丧失，从而损害其结构和极性，并影响其功能。我们表明，在非粘附条件下培养的单个大鼠 β 细胞会形成数量和大小随时间增加的胞质内液泡。我们表征了这些结构及其对 β 细胞功能的影响。