p-Benzoquinone (BQ) is a lignin-derived inhibitor to microbial strains. Unlike the furan inhibitors, p-benzoquinone is recalcitrant to traditional detoxification methods. This study shows a biological degradation of p-benzoquinone and a simultaneous D-lactic acid fermentation by an engineered Pediococcus acidilactici strain. The overexpression of an oxidoreductase gene ZMO1116 from Zymomonas mobilis encoding oxidoreductase was identified to improve the D-lactic acid fermentability of P. acidilactici against p-benzoquinone. The gene ZMO1116 was integrated into the genome of P. acidilactici and enabled the engineered P. acidilactici to convert p-benzoquinone into less toxic hydroquinone (HQ), resulting in the improved p-benzoquinone tolerance. Simultaneous saccharification and co-fermentation (SSCF) was conducted using the pretreated and biodetoxified corn stover containing p-benzoquinone, the D-lactic acid production of the engineered strain (123.8 g/L) was 21.4 % higher than the parental strain (102.0 g/L). This study provides a practical method on robust p-benzoquinone tolerance and efficient cellulosic chiral lactic acid fermentation from lignocellulose feedstock.

对苯醌 (BQ) 是一种木质素衍生的微生物菌株抑制剂。与呋喃抑制剂不同，对苯醌对传统的解毒方法有抵抗力。该研究显示了对苯醌的生物降解和由工程化的乳酸片球菌菌株同时进行的 D-乳酸发酵。氧化还原酶基因的过表达ZMO1116从运动发酵单胞编码氧化还原酶被鉴定为改善的d乳酸发酵乳酸片球菌对p醌。基因ZMO1116被整合到基因组中乳酸片球菌并使工程化的P. acidilactici能够将对苯醌转化为毒性较低的对苯二酚 (HQ)，从而提高对苯醌的耐受性。使用预处理和生物脱毒的含有对苯醌的玉米秸秆进行同步糖化和共发酵 (SSCF) ，工程菌株的 D-乳酸产量 (123.8 g/L) 比亲本菌株 (102.0 g) 高 21.4% /L)。该研究提供了一种实用的方法，可以从木质纤维素原料中获得强大的对苯醌耐受性和有效的纤维素手性乳酸发酵。