A robust and highly specific sensor based on electroactive molecularly imprinted polymer nanoparticles (nanoMIP) was developed. The nanoMIP tagged with a redox probe, combines both recognition and reporting capabilities. The developed nanoMIP replaces enzyme-mediator pairs used in traditional biosensors thus, offering enhanced molecular recognition for insulin, improving performance in complex biological samples, and yielding high stability. Also, most of existing sensors show poor performance after storage. To improve costs of the logistics and avoid the need of cold storage in the chain supply, we developed an alternative to biorecognition system that relies on nanoMIP. NanoMIP were computationally designed using “in-silico” insulin epitope mapping and synthesized by solid phase polymerisation. The characterisation of the polymer nanoparticles was performed by transmission electron microscopy (TEM), dynamic light scattering (DLS), Fourier-transform Infrared (FT-IR) and surface plasmon resonance (SPR). The electrochemical sensor was developed by chemical immobilisation of the nanoMIP on screen printed platinum electrodes. The insulin sensor displayed satisfactory performances and reproducible results (RSD = 4.2%; n = 30) using differential pulse voltammetry (DPV) in the clinically relevant concentration range from 50 to 2000 pM. The developed nanoMIP offers the advantage of large number of specific recognition sites with tailored geometry, as the resultant, the sensor showed high sensitivity and selectivity to insulin with a limit of detection (LOD) of 26 and 81 fM in buffer and human plasma, respectively, confirming the practical application for point of care monitoring. Moreover, the nanoMIP showed adequate storage stability of 168 days, demonstrating the robustness of sensor for several rounds of insulin analysis.

开发了一种基于电活性分子印迹聚合物纳米颗粒（nanoMIP）的功能强大且高度特异性的传感器。带有氧化还原探针的nanoMIP结合了识别和报告功能。开发的nanoMIP取代了传统生物传感器中使用的酶-介体对，从而增强了胰岛素的分子识别能力，改善了复杂生物样品的性能，并具有很高的稳定性。同样，大多数现有传感器在存储后显示出较差的性能。为了提高物流成本并避免在链式供应中需要冷藏，我们开发了一种替代生物识别系统的方法，该系统依赖于nanoMIP。NanoMIP使用“计算机内”胰岛素表位作图计算设计，并通过固相聚合合成。聚合物纳米颗粒的表征通过透射电子显微镜（TEM），动态光散射（DLS），傅立叶变换红外（FT-IR）和表面等离子体共振（SPR）进行。电化学传感器是通过将nanoMIP化学固定在丝网印刷的铂电极上而开发的。胰岛素传感器表现出令人满意的性能和可重复的结果（RSD = 4.2％；n  = 30）使用临床相关浓度范围为50至2000 pM的差分脉冲伏安法（DPV）。研发的nanoMIP具有大量具有定制几何形状的特定识别位点的优势，因此，该传感器对胰岛素表现出高灵敏度和选择性，在缓冲液和人血浆中的检测限（LOD）分别为26和81 fM ，证实了现场监测的实际应用。此外，nanoMIP表现出足够的168天储存稳定性，证明了传感器在几轮胰岛素分析中的坚固性。