The aim of this study was to investigate effects in dogs of ultrasonic waves on the ovarian germinal tissues. There was assignment of ovaries of each dog being a control (right ovary) or treated (left ovary) tissue, regardless of the estrous cycle status. For the treated ovary, the ultrasonic probe was set at a frequency of 1 MHz and power of 1.5 W/cm² and placed on the skin at a location in close proximity to the left ovary that was predetermined using ultrasonography. Subsequently there was a focusing of the waves for 5 min on the tissues of the treated ovary. The treatment was repeated three times at 48 -h intervals. There was placement of the probe at the same proximity to the control ovary; however, the probe was not activated. Ovariectomies for histological examination were performed 30 (five dogs) and 60 (five dogs) days following the ultrasonic treatments. Histopathological examination results indicated there was a reduction in numbers of primordial follicles of four dogs and oocyte degeneration in the Graafian and primordial follicles of five dogs. In most dogs, the degeneration of the granulosa cells of the Graafian follicle was observed in the treated ovaries. The results indicate the treatment of ovaries with transabdominal ultrasonic waves at a 1 MHz frequency, and 1.5 W/cm² power for three times with each treatment lasting for 5 min at a 48-h interval, did not result in complete ablation of the germinal tissues of the ovary during the 2-month period subsequent to treatment.

本研究的目的是研究超声波对犬卵巢生发组织的影响。无论发情周期状态如何，每只狗的卵巢都被指定为对照（右卵巢）或治疗（左卵巢）组织。对于处理过的卵巢，超声探头的频率设置为 1 MHz，功率为 1.5 W/cm ²并放置在靠近左侧卵巢的皮肤上，该位置是使用超声检查预先确定的。随后将波聚焦在处理过的卵巢组织上 5 分钟。以48小时的间隔重复处理3次。将探针放置在与对照卵巢相同的位置；然而，探测器没有被激活。用于组织学检查的卵巢切除术在超声治疗后 30（五只狗）和 60（五只狗）天进行。组织病理学检查结果表明，4只狗的原始卵泡数量减少，Graafian和5只狗的原始卵泡出现卵母细胞变性。在大多数狗中，在处理过的卵巢中观察到 Graafian 卵泡的颗粒细胞退化。²功率 3 次，每次治疗持续 5 分钟，间隔 48 小时，在治疗后的 2 个月期间未导致卵巢生发组织完全消融。