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Ultrasensitive electrochemical biosensor for detection of microRNA-155 as a breast cancer risk factor
Analytica Chimica Acta ( IF 6.2 ) Pub Date : 2020-11-01 , DOI: 10.1016/j.aca.2020.08.039
Fatemeh Hakimian , Hedayatollah Ghourchian

Due to the stability of microRNAs (miRNAs) in serum and other body fluids, they are known as promising cancer biomarkers. Recent studies have indicated higher expression of miRNA-155 (miR-155) in patients with breast cancer compared to healthy people. In the present report, a rapid and sensitive electrochemical biosensor has been developed for detection of miR-155 as a breast cancer risk factor. At first, a thiolated probe was immobilized on the gold electrode surface. Then, the target (miR-155) was exposed to the probe. In the next step, the positively charged polyethyleneimine-silver nanoparticles as electroactive labels were absorbed onto the negatively charged probe-target hybrid. In the third step, the anodic peak current which was produced due to the oxidation of silver nanoparticles was recorded as the electrochemical signal. The designed biosensor provided an ultrasensitive method for the detection of miR-155 with the detection limit of 20 zmol and a wide linear range from 2 × 10-20 to 2 × 10-12 mol. Moreover, the biosensor was able to detect miR-155 in real serum samples with satisfactory results.

中文翻译:

用于检测作为乳腺癌危险因素的 microRNA-155 的超灵敏电化学生物传感器

由于血清和其他体液中微小 RNA (miRNA) 的稳定性,它们被认为是有前途的癌症生物标志物。最近的研究表明,与健康人相比,乳腺癌患者中 miRNA-155 (miR-155) 的表达更高。在本报告中,开发了一种快速灵敏的电化学生物传感器,用于检测作为乳腺癌危险因素的 miR-155。首先,将硫醇化探针固定在金电极表面。然后,目标(miR-155)暴露于探针。在下一步中,带正电的聚乙烯亚胺-银纳米颗粒作为电活性标记被吸附到带负电的探针-靶标杂交体上。在第三步中,由于银纳米颗粒氧化而产生的阳极峰值电流被记录为电化学信号。所设计的生物传感器为 miR-155 的检测提供了一种超灵敏的方法,其检测限为 20 zmol,线性范围为 2 × 10-20 至 2 × 10-12 mol。此外,该生物传感器能够检测真实血清样品中的 miR-155,结果令人满意。
更新日期:2020-11-01
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