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Effect of Vanadium on Growth, Photosynthesis, Reactive Oxygen Species, Antioxidant Enzymes, and Cell Death of Rice
Journal of Soil Science and Plant Nutrition ( IF 3.9 ) Pub Date : 2020-08-27 , DOI: 10.1007/s42729-020-00330-x
Muhammad Mohsin Altaf , Xiao-ping Diao , Atique ur Rehman , Muhammad Imtiaz , Awais Shakoor , Muhammad Ahsan Altaf , Haseeb Younis , Pengcheng Fu , Muhammad Usman Ghani

Vanadium (V) as minor concentration is present in various plants and extensively found in soils. The current study was established to assess the response of rice seedlings to different V concentrations and also investigated its toxic effect on growth, photosynthetic assimilation, relative chlorophyll content, SPAD index, ion leakage, enzyme activities, hydrogen peroxide (H2O2), and cell death. The rice seeds were sown in Petri dishes for 8 days, and after that, rice seedlings were grown hydroponically in a climate-controlled growth chamber. After 15 days of V-treatment, antioxidant enzyme activities, H2O2, protein contents, photosynthetic assimilation, relative chlorophyll content, and cell death were determined by utilizing the Spectrophotometer (Lambda 25 UV/VIS Spectrophotometer), and V accumulation (roots and shoots) was determined by GFAAS (GTA 120). The obtained results showed that all V concentrations significantly decreased the biomass (dry and fresh) and root growth as a result of the reduction in total root length, root tips, root fork, root surface area, and root crossing, and V was more accumulated in roots than shoots. Besides this, enzymatic activities were significantly enhanced under V stress. The findings also confirmed that seedling exposed to V stress had lower tolerance indices, photosynthetic activity, and protein contents while the ion leakage was consistently increased by increasing the V concentrations. The viability of plant cells severely damaged in response to high V stress, and H2O2 induction might be responsible for cell death. Generally, all V doses had a drastic effect on enzyme activities and caused cell death of rice plans. Moreover, the current study demonstrated that V ≥ 35 mg L−1 caused damaging effects on rice plants.

中文翻译:

钒对水稻生长、光合作用、活性氧种类、抗氧化酶和细胞死亡的影响

钒 (V) 作为次要浓度存在于各种植物中,并广泛存在于土壤中。本研究旨在评估水稻幼苗对不同 V 浓度的响应,并研究其对生长、光合同化、相对叶绿素含量、SPAD 指数、离子泄漏、酶活性、过氧化氢 (H2O2) 和细胞死亡的毒性作用. 水稻种子在培养皿中播种 8 天,之后,水稻幼苗在气候控制的生长室中水培生长。V处理15天后,利用分光光度计(Lambda 25 UV/VIS分光光度计)测定抗氧化酶活性、H2O2、蛋白质含量、光合同化、相对叶绿素含量和细胞死亡,V 积累(根和芽)由 GFAAS (GTA 120) 确定。获得的结果表明,由于总根长、根尖、根叉、根表面积和根交叉的减少,所有 V 浓度显着降低了生物量(干和新鲜)和根生长,并且 V 积累更多在根比芽。除此之外,在 V 胁迫下酶活性显着增强。研究结果还证实,暴露于 V 胁迫的幼苗具有较低的耐受性指数、光合活性和蛋白质含量,而离子泄漏随着 V 浓度的增加而持续增加。植物细胞的活力因高 V 胁迫而严重受损,H2O2 诱导可能是细胞死亡的原因。一般来说,所有 V 剂量对酶活性都有剧烈影响,并导致水稻计划的细胞死亡。此外,目前的研究表明,V ≥ 35 mg L-1 会对水稻造成破坏性影响。
更新日期:2020-08-27
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