Long non-coding RNA (LncRNA) LINC00525 has been shown to be upregulated in several human cancers and deduced to possess caner regulatory role. The regulation of molecular mechanics of human glioma by lncRNA-LINC00525 through microRNA sponging in glioma is elusive. The lncRNA-LINC00525 showed significant (P < 0.05) upregulation in glioma cancer cells. The upregulation of lncRNA-LINC00525 was upto 6.6-fold in glioma cells relative to the normal cells. Knockdown of lncRNA-LINC00525 significantly declined the proliferation of the glioma cancer cells. Additionally, the colony formation was inhibited by around 60% in glioma cells. The wound healing and transwell assays revealed significant (P < 0.05) inhibition of migration and invasion potential under lncRNA-LINC00525 knockdown. The western blotting study of biomarkers of epithelial to mesenchymal transition (EMT) revealed that lncRNA-LINC00525 gene silencing reduced the expression of mesenchymal molecular markers but increased the protein levels of epithelial markers. miR-338-3p was predicted to be interacting with lncRNA-LINC00525 in glioma and was shown to mediated the regulatory role of lncRNA-LINC00525. Taken together, the results of present study are supportive of the prognostic applicability of lncRNA-LINC00525 against human glioma together with its therapeutic potential against the said malignancy.

中文翻译：

---

长链非编码 RNA LINC00525 通过海绵化 miR-338-3p 调节人神经胶质瘤细胞的增殖和上皮间质转化。

长链非编码 RNA (LncRNA) LINC00525 已被证明在几种人类癌症中被上调，并被推断具有癌症调节作用。lncRNA-LINC00525 通过神经胶质瘤中的 microRNA 海绵作用对人神经胶质瘤分子力学的调控尚不清楚。lncRNA-LINC00525 在神经胶质瘤癌细胞中表现出显着 (P < 0.05) 上调。相对于正常细胞，lncRNA-LINC00525 在神经胶质瘤细胞中的上调高达 6.6 倍。lncRNA-LINC00525 的敲低显着降低了神经胶质瘤癌细胞的增殖。此外，胶质瘤细胞的集落形成被抑制了约 60%。伤口愈合和 transwell 测定显示，在 lncRNA-LINC00525 敲低下，迁移和侵袭潜力受到显着 (P < 0.05) 抑制。上皮间充质转化（EMT）生物标志物的蛋白质印迹研究表明，lncRNA-LINC00525基因沉默降低了间充质分子标志物的表达，但增加了上皮标志物的蛋白质水平。预测 miR-338-3p 与神经胶质瘤中的 lncRNA-LINC00525 相互作用，并显示介导 lncRNA-LINC00525 的调节作用。总之，本研究的结果支持 lncRNA-LINC00525 对人类神经胶质瘤的预后适用性及其对所述恶性肿瘤的治疗潜力。预测 miR-338-3p 与神经胶质瘤中的 lncRNA-LINC00525 相互作用，并显示介导 lncRNA-LINC00525 的调节作用。总之，本研究的结果支持 lncRNA-LINC00525 对人类神经胶质瘤的预后适用性及其对所述恶性肿瘤的治疗潜力。预测 miR-338-3p 与神经胶质瘤中的 lncRNA-LINC00525 相互作用，并显示介导 lncRNA-LINC00525 的调节作用。总之，本研究的结果支持 lncRNA-LINC00525 对人类神经胶质瘤的预后适用性及其对所述恶性肿瘤的治疗潜力。