Antibodies play a crucial role in basic research and clinical decision-making. However, there are no standardized algorithms or guidelines to ensure their accuracy and validity. There have been efforts to generate consensus, but, with the exception of clinical labs, antibody validation remains variable in the literature and sometimes in clinical practice. Here we focus on immunohistochemistry, an example of a scientific and clinical tool where validation of antibodies is critical. We describe a protocol that we use to validate antibodies specifically for immunohistochemistry, including some of the pillars of antibody validation from Uhlen et al. 2016, as an example of a rigorous approach to build antibody-based tests for both basic and translational science labs and for the clinic.

中文翻译：

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组织载玻片上蛋白质表达的抗体验证：免疫组织化学方案。

抗体在基础研究和临床决策中起着至关重要的作用。但是，没有标准化的算法或指南来确保其准确性和有效性。已经努力达成共识，但是，除了临床实验室，抗体验证在文献中，有时在临床实践中仍然存在差异。在这里，我们专注于免疫组织化学，这是一种科学和临床工具的例子，其中抗体的验证至关重要。我们描述了一个协议，我们用来验证专门用于免疫组织化学的抗体，包括 Uhlen等人的抗体验证的一些支柱。2016 年，作为为基础和转化科学实验室以及临床建立基于抗体的测试的严格方法的一个例子。