Evaluation of Cysteine Protease C of Leishmania donovani in comparison with Glycoprotein 63 and Elongation Factor-1α for diagnosis of human visceral leishmaniasis and for post-treatment follow-up response.,Journal of Clinical Microbiology

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Evaluation of Cysteine Protease C of Leishmania donovani in comparison with Glycoprotein 63 and Elongation Factor-1α for diagnosis of human visceral leishmaniasis and for post-treatment follow-up response.
Journal of Clinical Microbiology ( IF 9.4 ) Pub Date : 2020-10-21 , DOI: 10.1128/jcm.00213-20
Nicky Didwania ₁ , Sarfaraz Ahmad Ejazi ₁ , Rudra Chhajer ₁ , Abdus Sabur ₁ , Saumyabrata Mazumder ₁ , Mohd Kamran ₁ , Raunak Kar ₁ , Krishna Pandey ₂ , Vidya Nand Ravi Das ₂ , Pradeep Das ₃ , Mehebubar Rahaman ₄ , Rama Prosad Goswami ₄ , Nahid Ali ₅

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Visceral leishmaniasis (VL) is a threat in many developing countries. Much effort has been put to eliminating this disease, for which serodiagnosis remains the mainstay for VL control programs. New and improved antigens as diagnostic candidates are required, though, as the available antigens fail to demonstrate equal optimum performance in all areas of endemicity. Moreover, these diagnoses are dependent on invasive serum sampling. In the current study, we cloned and expressed Leishmania donovani cysteine protease C (CPC) and evaluated its diagnostic and test-of-cure possibilities by detecting the antibody levels in human serum and urine through ELISA and immunoblot assays. Two immunodominant antigens, recombinant glycoprotein 63 (GP63) and elongation factor 1α (EF1α), identified earlier by our group, were also assessed by employing human serum and urine samples. Of these three antigens in ELISAs, CPC demonstrated the highest sensitivities of 98.15% and 96% positive testing in serum and urine of VL patients, respectively. Moreover, CPC yielded 100% specificity with serum and urine of nonendemic healthy controls compared to GP63 and EF1α. Urine samples were found to be more specific than serum for distinguishing endemic healthy controls and other diseases by means of all three antigens. In all cases, CPC gave the most promising results. Unlike serum, urine tests demonstrated a significant decrease in antibody levels for CPC, GP63, and EF1α after 6 months of treatment. The diagnostic and test-of-cure performances of CPC in the immunoblot assay were found to be better than those of GP63 and EF1α. In conclusion, CPC, followed by GP63 and EF1α, may be utilized as candidates for diagnosis of VL and to assess treatment response.

更新日期：2020-10-27

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