Staphylococcus aureus is a bacterial pathogen and one of the leading causes of healthcare‐acquired infections in the world. The growing antibiotic resistance of S. aureus obliges us to search for new drugs and treatments. As the majority of antibiotics target the ribosome, knowledge of its detailed structure is crucial for drug development. Here, we report the cryo‐EM reconstruction at 3.2 Å resolution of the S. aureus ribosome with P‐site tRNA, messenger RNA, and 10 RNA modification sites previously not assigned or visualized. The resulting model is the most precise and complete high‐resolution structure to date of the S. aureus 70S ribosome with functional ligands.

中文翻译：

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人类病原体金黄色葡萄球菌核糖体功能复合物的冷冻电镜结构，分辨率为 3.2 Å

金黄色葡萄球菌是一种细菌病原体，也是全球医疗保健获得性感染的主要原因之一。金黄色葡萄球菌日益增长的抗生素耐药性迫使我们寻找新的药物和治疗方法。由于大多数抗生素针对核糖体，了解其详细结构对于药物开发至关重要。在这里，我们报告了具有 P 位点 tRNA、信使 RNA 和 10 个以前未分配或可视化的 RNA 修饰位点的金黄色葡萄球菌核糖体在 3.2 Å 分辨率下的冷冻电镜重建。由此产生的模型是迄今为止具有功能配体的金黄色葡萄球菌 70S 核糖体的最精确和最完整的高分辨率结构。